Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997013040> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1997013040 endingPage "328" @default.
- W1997013040 startingPage "322" @default.
- W1997013040 abstract "Previously, we described GFP-spectrin, a membrane-localized derivative of the green fluorescent protein that can be employed as a marker during the simultaneous identification of transfected cells and cell cycle analysis by flow cytometry (Kalejta et al., Cytometry 29: 286-291, 1997). A membrane-anchored GFP fusion protein is necessary because the ethanol permeabilization step required to achieve efficient propidium iodide staining allows cytoplasmic GFP to leach out of the cell. However, viable cells expressing GFP-spectrin are not as bright as cells expressing cytoplasmic GFP and their fluorescence intensity is further diminished after ethanol treatment. Here, we demonstrate that the fluorescence intensity of cells expressing an integral membrane GFP fusion protein (Us9-GFP) is similar to that of cells expressing cytoplasmic GFP and is quantitatively maintained in cells after ethanol treatment. By allowing an accurate assessment of the expression level of GFP, Us9-GFP allows a more precise analysis of the effects of a cotransfected plasmid on the cell cycle and thus represents an improvement upon the original membrane-associated GFP fusion proteins employed in this assay." @default.
- W1997013040 created "2016-06-24" @default.
- W1997013040 creator A5052201128 @default.
- W1997013040 creator A5065735862 @default.
- W1997013040 creator A5071460138 @default.
- W1997013040 creator A5072190727 @default.
- W1997013040 date "1999-04-01" @default.
- W1997013040 modified "2023-09-25" @default.
- W1997013040 title "An Integral Membrane Green Fluorescent Protein Marker, Us9-GFP, Is Quantitatively Retained in Cells during Propidium Iodide-Based Cell Cycle Analysis by Flow Cytometry" @default.
- W1997013040 cites W1015466433 @default.
- W1997013040 cites W1518667274 @default.
- W1997013040 cites W1762826881 @default.
- W1997013040 cites W1799140375 @default.
- W1997013040 cites W1984751884 @default.
- W1997013040 cites W1999175477 @default.
- W1997013040 cites W2005782605 @default.
- W1997013040 cites W2011229368 @default.
- W1997013040 cites W2013005834 @default.
- W1997013040 cites W2017753207 @default.
- W1997013040 cites W2026896701 @default.
- W1997013040 cites W2028204795 @default.
- W1997013040 cites W2059500251 @default.
- W1997013040 cites W2100056890 @default.
- W1997013040 cites W2124665801 @default.
- W1997013040 cites W2127068865 @default.
- W1997013040 cites W2176744910 @default.
- W1997013040 cites W22767932 @default.
- W1997013040 cites W4233808990 @default.
- W1997013040 doi "https://doi.org/10.1006/excr.1999.4427" @default.
- W1997013040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10094838" @default.
- W1997013040 hasPublicationYear "1999" @default.
- W1997013040 type Work @default.
- W1997013040 sameAs 1997013040 @default.
- W1997013040 citedByCount "58" @default.
- W1997013040 countsByYear W19970130402012 @default.
- W1997013040 countsByYear W19970130402013 @default.
- W1997013040 countsByYear W19970130402014 @default.
- W1997013040 countsByYear W19970130402015 @default.
- W1997013040 countsByYear W19970130402016 @default.
- W1997013040 countsByYear W19970130402018 @default.
- W1997013040 countsByYear W19970130402019 @default.
- W1997013040 countsByYear W19970130402020 @default.
- W1997013040 countsByYear W19970130402022 @default.
- W1997013040 crossrefType "journal-article" @default.
- W1997013040 hasAuthorship W1997013040A5052201128 @default.
- W1997013040 hasAuthorship W1997013040A5065735862 @default.
- W1997013040 hasAuthorship W1997013040A5071460138 @default.
- W1997013040 hasAuthorship W1997013040A5072190727 @default.
- W1997013040 hasConcept C104317684 @default.
- W1997013040 hasConcept C123894998 @default.
- W1997013040 hasConcept C142613039 @default.
- W1997013040 hasConcept C153911025 @default.
- W1997013040 hasConcept C190283241 @default.
- W1997013040 hasConcept C2775934118 @default.
- W1997013040 hasConcept C31573885 @default.
- W1997013040 hasConcept C40767141 @default.
- W1997013040 hasConcept C54009773 @default.
- W1997013040 hasConcept C553184892 @default.
- W1997013040 hasConcept C55493867 @default.
- W1997013040 hasConcept C86803240 @default.
- W1997013040 hasConcept C95444343 @default.
- W1997013040 hasConceptScore W1997013040C104317684 @default.
- W1997013040 hasConceptScore W1997013040C123894998 @default.
- W1997013040 hasConceptScore W1997013040C142613039 @default.
- W1997013040 hasConceptScore W1997013040C153911025 @default.
- W1997013040 hasConceptScore W1997013040C190283241 @default.
- W1997013040 hasConceptScore W1997013040C2775934118 @default.
- W1997013040 hasConceptScore W1997013040C31573885 @default.
- W1997013040 hasConceptScore W1997013040C40767141 @default.
- W1997013040 hasConceptScore W1997013040C54009773 @default.
- W1997013040 hasConceptScore W1997013040C553184892 @default.
- W1997013040 hasConceptScore W1997013040C55493867 @default.
- W1997013040 hasConceptScore W1997013040C86803240 @default.
- W1997013040 hasConceptScore W1997013040C95444343 @default.
- W1997013040 hasIssue "1" @default.
- W1997013040 hasLocation W19970130401 @default.
- W1997013040 hasLocation W19970130402 @default.
- W1997013040 hasOpenAccess W1997013040 @default.
- W1997013040 hasPrimaryLocation W19970130401 @default.
- W1997013040 hasRelatedWork W2035303685 @default.
- W1997013040 hasRelatedWork W2164545422 @default.
- W1997013040 hasRelatedWork W2354688522 @default.
- W1997013040 hasRelatedWork W2364595522 @default.
- W1997013040 hasRelatedWork W2368863504 @default.
- W1997013040 hasRelatedWork W2373087578 @default.
- W1997013040 hasRelatedWork W2374821441 @default.
- W1997013040 hasRelatedWork W2376878577 @default.
- W1997013040 hasRelatedWork W2382432990 @default.
- W1997013040 hasRelatedWork W2410997255 @default.
- W1997013040 hasVolume "248" @default.
- W1997013040 isParatext "false" @default.
- W1997013040 isRetracted "false" @default.
- W1997013040 magId "1997013040" @default.
- W1997013040 workType "article" @default.