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• W1997044547 abstract "Response surface methodology based on a central composite rotatable design has been successfully used to model and optimize biochemical and biotechnological processes. Heat treatment could have an effect on beta-lactoglobulin (β-Lg) and lead to allergic reaction. To reduce this phenomenon, liposomes were used as carriers in this research. The mass ratio of phosphatidylcholine and cholesterol (2.5–12.5), β-Lg concentration (2.5–12.5 mg mL−1), sonication time (5–25, min) and temperature (25–40 °C) were selected as independent variables with encapsulation efficiency as a dependent variable. For each response, a second-order polynomial model was developed using multiple linear regression analysis. Applying a desirability function method the optimum parameters were: phosphatidylcholine to cholesterol ratio of 8.05, β-Lg concentration of 9.09 mg mL−1, sonication time of 17.71 min and temperature of 30 °C. The particle size and encapsulation efficiency were found to be 189 nm and 61.55%, respectively. Furthermore, the in vitro stability of β-Lg nano-liposomes in simulated gastrointestinal juice was evaluated. The nano-liposomes showed an acceptable stability in simulated gastrointestinal juice at 37 °C for 4 h." @default.
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• W1997044547 date "2014-01-01" @default.
• W1997044547 modified "2023-09-26" @default.
• W1997044547 title "Response surface methodology for the optimization of beta-lactoglobulin nano-liposomes" @default.
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• W1997044547 doi "https://doi.org/10.1039/c3fo60476d" @default.
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