Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997091574> ?p ?o ?g. }
- W1997091574 endingPage "41" @default.
- W1997091574 startingPage "29" @default.
- W1997091574 abstract "We have used astrocyte-conditioned medium (ACM) to promote the transdifferentiation of bovine chromaffin cells and study modifications in the exocytotic process when these cells acquire a neuronal phenotype. In the ACM-promoted neuronal phenotype, secretory vesicles and intracellular Ca2+ rise were preferentially distributed in the neurite terminals. Using amperometry, we observed that the exocytotic events also occurred mainly in the neurite terminals, wherein the individual exocytotic events had smaller quantal size than in undifferentiated cells. Additionally, duration of pre-spike current was significantly shorter, suggesting that ACM also modifies the fusion pore stability. After long exposure (7-9 days) to ACM, the kinetics of catecholamine release from individual vesicles was markedly accelerated. The morphometric analysis of vesicle diameters suggests that the rapid exocytotic events observed in neurites of ACM-treated cells correspond to the exocytosis of large dense-core vesicles (LDCV). On the other hand, experiments performed in EGTA-loaded cells suggest that ACM treatment promotes a better coupling between voltage-gated calcium channels (VGCC) and LDCV. Thus, our findings reveal that ACM promotes a neuronal phenotype in chromaffin cells, wherein the exocytotic kinetics is accelerated. Such rapid exocytosis mode could be caused at least in part by a better coupling between secretory vesicles and VGCC." @default.
- W1997091574 created "2016-06-24" @default.
- W1997091574 creator A5009961687 @default.
- W1997091574 creator A5034917115 @default.
- W1997091574 creator A5036582212 @default.
- W1997091574 creator A5048185569 @default.
- W1997091574 creator A5048393261 @default.
- W1997091574 creator A5060427202 @default.
- W1997091574 creator A5074769045 @default.
- W1997091574 creator A5080519673 @default.
- W1997091574 creator A5090920011 @default.
- W1997091574 date "2006-08-02" @default.
- W1997091574 modified "2023-10-16" @default.
- W1997091574 title "A rapid exocytosis mode in chromaffin cells with a neuronal phenotype" @default.
- W1997091574 cites W101355125 @default.
- W1997091574 cites W1509155252 @default.
- W1997091574 cites W1534488804 @default.
- W1997091574 cites W1541657072 @default.
- W1997091574 cites W1564650596 @default.
- W1997091574 cites W1599906730 @default.
- W1997091574 cites W1671421450 @default.
- W1997091574 cites W1853725381 @default.
- W1997091574 cites W1965893090 @default.
- W1997091574 cites W1970868557 @default.
- W1997091574 cites W1973193956 @default.
- W1997091574 cites W1982944462 @default.
- W1997091574 cites W1987368066 @default.
- W1997091574 cites W1987386100 @default.
- W1997091574 cites W1990171785 @default.
- W1997091574 cites W1991285925 @default.
- W1997091574 cites W1995784304 @default.
- W1997091574 cites W1996618398 @default.
- W1997091574 cites W2003380714 @default.
- W1997091574 cites W2003390216 @default.
- W1997091574 cites W2010579691 @default.
- W1997091574 cites W2016173046 @default.
- W1997091574 cites W2016738403 @default.
- W1997091574 cites W2017847991 @default.
- W1997091574 cites W2020249698 @default.
- W1997091574 cites W2029216493 @default.
- W1997091574 cites W2033067408 @default.
- W1997091574 cites W2035070093 @default.
- W1997091574 cites W2040913663 @default.
- W1997091574 cites W2042332532 @default.
- W1997091574 cites W2044030959 @default.
- W1997091574 cites W2045651022 @default.
- W1997091574 cites W2051340998 @default.
- W1997091574 cites W2058595266 @default.
- W1997091574 cites W2058856551 @default.
- W1997091574 cites W2059171611 @default.
- W1997091574 cites W2059994807 @default.
- W1997091574 cites W2063272994 @default.
- W1997091574 cites W2067800529 @default.
- W1997091574 cites W2072925847 @default.
- W1997091574 cites W2076776451 @default.
- W1997091574 cites W2080954023 @default.
- W1997091574 cites W2083620477 @default.
- W1997091574 cites W2087758815 @default.
- W1997091574 cites W2092593123 @default.
- W1997091574 cites W2094129437 @default.
- W1997091574 cites W2095284617 @default.
- W1997091574 cites W2113286858 @default.
- W1997091574 cites W2117602113 @default.
- W1997091574 cites W2122776408 @default.
- W1997091574 cites W2130020417 @default.
- W1997091574 cites W2139539061 @default.
- W1997091574 cites W2154649008 @default.
- W1997091574 cites W2171919992 @default.
- W1997091574 cites W2399172744 @default.
- W1997091574 doi "https://doi.org/10.1111/j.1471-4159.2006.04080.x" @default.
- W1997091574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16889641" @default.
- W1997091574 hasPublicationYear "2006" @default.
- W1997091574 type Work @default.
- W1997091574 sameAs 1997091574 @default.
- W1997091574 citedByCount "18" @default.
- W1997091574 countsByYear W19970915742013 @default.
- W1997091574 countsByYear W19970915742014 @default.
- W1997091574 countsByYear W19970915742015 @default.
- W1997091574 countsByYear W19970915742016 @default.
- W1997091574 countsByYear W19970915742017 @default.
- W1997091574 countsByYear W19970915742019 @default.
- W1997091574 countsByYear W19970915742022 @default.
- W1997091574 crossrefType "journal-article" @default.
- W1997091574 hasAuthorship W1997091574A5009961687 @default.
- W1997091574 hasAuthorship W1997091574A5034917115 @default.
- W1997091574 hasAuthorship W1997091574A5036582212 @default.
- W1997091574 hasAuthorship W1997091574A5048185569 @default.
- W1997091574 hasAuthorship W1997091574A5048393261 @default.
- W1997091574 hasAuthorship W1997091574A5060427202 @default.
- W1997091574 hasAuthorship W1997091574A5074769045 @default.
- W1997091574 hasAuthorship W1997091574A5080519673 @default.
- W1997091574 hasAuthorship W1997091574A5090920011 @default.
- W1997091574 hasConcept C113246987 @default.
- W1997091574 hasConcept C12554922 @default.
- W1997091574 hasConcept C130316041 @default.
- W1997091574 hasConcept C134018914 @default.
- W1997091574 hasConcept C148785051 @default.
- W1997091574 hasConcept C169760540 @default.