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- W1997131885 abstract "Abstract The development of methods for the isolation of nucleoli (1–3) from mammalian liver and tumor cells has provided an opportunity for the study of the biosynthetic reactions that occur in these nuclear organelles. Evidence that the rate of turnover of RNA in the nucleolus is very high has been obtained by autoradiographic studies (4) and more recently by experiments on uptake of the isotope of labeled orotic acid into RNA of nucleoli of livers of control and thioacetamide-treated rats (5,6). Maggio et al (3) have also studied the uptake of 32P into nucleoli of guinea pig livers. In the present experiments, conditions and cofactor requirements for incorporation of the isotope of UTP-2-14C into nucleolar RNA were determined. Along with the inhibitory effects of Actinomycin D and DNase, these experiments provide supporting evidence that the biosynthesis of RNA in the nucleolus is DNA-primed. Inasmuch as the product is largely GC-type RNA (3,7), the data suggested that isolated DNA segments rich in GC are derepressed for biosynthesis of nucleolar components." @default.
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- W1997131885 date "1964-01-01" @default.
- W1997131885 modified "2023-09-23" @default.
- W1997131885 title "Labeling of RNA of isolated nucleoli with UTP-14C" @default.
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- W1997131885 doi "https://doi.org/10.1016/0006-291x(64)90245-1" @default.
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