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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997149716> ?p ?o ?g. }

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• W1997149716 abstract "We optimized a previously established two-plasmid system for the identification of Staphylococcus aureus promoters that are recognized by the alternative transcription factor sigma(B). The method allowed the identification of 18 S. aureus sigma(B)-dependent promoters, 12 of which are reported here for the first time to be sigma(B)-dependent. S1-nuclease mapping of the respective transcriptional start points revealed that all the promoters contained sequences exhibiting high similarity to the consensus sequence of Bacillus subtilis sigma(B)-dependent promoters. The promoters governed expression of genes encoding proteins proposed to be involved in various cellular functions, including the stress response genes and virulence-associated clfA gene for fibrinogen-binding clumping factor. Comparison of the nucleotide sequences upstream of the identified transcription start points identified a sigma(B) consensus promoter (GttTaa-N(12-15)-gGGTAt) that is highly homologous to that of sigma(B) of B. subtilis." @default.
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• W1997149716 date "2004-03-01" @default.
• W1997149716 modified "2023-10-07" @default.
• W1997149716 title "Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containing<i>Staphylococcus aureus</i>alternative sigma factor σ^B" @default.
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• W1997149716 doi "https://doi.org/10.1016/s0378-1097(04)00063-1" @default.
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