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• W1997155312 abstract "Growth kinetics of Pseudomonas putida (ATCC 49451) in cometabolism of phenol and 4-chlorophenol (4-cp) in the presence of sodium glutamate (SG) were studied. In the ternary substrate mixture, phenol and SG are growth substrates while 4-cp is a nongrowth substrate. Cell growth on phenol was found to follow Andrews kinetics and cells displayed substrate inhibition pattern on sodium glutamate in the range of 0–4 g L−1 as well. A cell growth model for the ternary substrate system was established based on a simplified cell growth mechanism and subsequently modified by experimental results. Model analysis over a wide range of substrate concentrations shows that the inhibition of SG is much larger than phenol at low phenol concentrations (⩽200 mg L−1) while phenol exerts dominant inhibition on cell growth at higher phenol concentrations (⩾600 mg L−1). The nongrowth substrate, 4-cp, inhibits cell growth mainly through inactivation of cells (cell decay) and competitive inhibition to cell growth on phenol. In the absence of SG, 4-cp retards cell growth severely and cells cannot grow at 250 mg L−1 4-cp. Addition of sodium glutamate, however, greatly attenuates the toxicity of 4-cp and supports cell growth at 4-cp concentration higher than 250 mg L−1. By using the proposed cell growth model, we were able to optimize the amount of SG needed to enhance cell growth rate and validate model predictions against experimental data. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 437–447, 2000." @default.
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• W1997155312 date "2000-05-20" @default.
• W1997155312 modified "2023-10-12" @default.
• W1997155312 title "Growth kinetics ofPseudomonas putida in cometabolism of phenol and 4-chlorophenol in the presence of a conventional carbon source" @default.
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• W1997155312 doi "https://doi.org/10.1002/(sici)1097-0290(20000520)68:4<437::aid-bit9>3.0.co;2-g" @default.
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