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- W1997159904 abstract "When bacteriophage λpgal8 DNA is transcribed in a purified in vitro system by E. coli RNA polymerase (nucleoside triphosphate: RNA nucleotidyl-transferase, EC 2.7.7.6), several major transcripts are synthesized. We have investigated transcriptional termination of one of these transcripts, the 4S, or “oop” RNA. Analysis by two-dimensional “fingerprinting” of Tl oligonucleotides reveals that transcription of the 4S RNA terminates at a specific site on the λpgal8 DNA template, tL′ with an efficiency of approximately 80Z, i.e. 20% of transcripts are extended into larger RNAs. Addition of the E. coli protein rho to our transcription reactions has two effects: a) the efficiency of termination at the tL′ site is increased to 100%; b) the number of AS transcripts synthesized is increased by greater than 5-fold. Rho appears to stimulate 4S RNA synthesis by facilitating more rapid release of RNA polymerase from the tL′ termination site." @default.
- W1997159904 created "2016-06-24" @default.
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- W1997159904 date "1977-01-01" @default.
- W1997159904 modified "2023-09-26" @default.
- W1997159904 title "Transcription in vitro of bacteriophage lambda 4S RNA: studies on termination and rho protein" @default.
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- W1997159904 doi "https://doi.org/10.1093/nar/4.4.827" @default.
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