Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997162244> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1997162244 endingPage "742" @default.
- W1997162244 startingPage "737" @default.
- W1997162244 abstract "The essential oil from mature leaves of flowering peppermint (Mentha piperita L.) contains up to 15% (—)-menthyl acetate, and leaf discs converted exogenous (—)-[G-3H]menthol into this ester in approximately 15% yield of the incorporated precursor. Leaf extracts catalyzed the acetyl coenzyme A-dependent acetylation of (—)-[G-3H]menthol and the product of this transacetylase reaction was identified by radiochromatographic techniques. Transacetylase activity was located mainly in the 100,000g supernatant fraction, and the preparation was partially purified by combination of Sephadex G-100 gel filtration and chromatography on O-diethylaminoethyl-cellulose. The transacetylase had a molecular weight of about 37,000 as judged by Sephadex G-150 gel filtration, and a pH optimum near 9. The apparent Km and velocity for (—)-menthol were 0.3 mm and 16 nmol/hr· mg of protein, respectively. The saturation curve for acetyl coenzyme A was sigmoidal, showing apparent saturation near 0.1 mm. Dithioerythritol was required for maximum activity and stability of the enzyme, and the enzyme was inhibited by thiol directed reagents such as p-hydroxymercuribenzoate. Diisopropylfluorophosphate also inhibited transacylation suggesting the involvement of a serine residue in catalysis. The transacylase was highly specific for acetyl coenzyme A; propionyl coenzyme A and butyryl coenzyme A were not nearly as efficient as acyl donors (11% and 2%, respectively). However, the enzyme was much less selective with regard to the alcohol substrate, suggesting that the nature of the acetate ester synthesized in mint is more dependent on the type of alcohol available than on the specificity of the transacetylase. This is the first report on an enzyme involved in monoterpenol acetylation in plants. A very similar enzyme, catalyzing this key reaction in the metabolism of menthol, was also isolated from the flowers of peppermint." @default.
- W1997162244 created "2016-06-24" @default.
- W1997162244 creator A5007103570 @default.
- W1997162244 creator A5060790939 @default.
- W1997162244 date "1978-05-01" @default.
- W1997162244 modified "2023-09-27" @default.
- W1997162244 title "Metabolism of Monoterpenes" @default.
- W1997162244 cites W1233505759 @default.
- W1997162244 cites W1567966142 @default.
- W1997162244 cites W1577385599 @default.
- W1997162244 cites W1585864199 @default.
- W1997162244 cites W1775749144 @default.
- W1997162244 cites W1983231732 @default.
- W1997162244 cites W1990394053 @default.
- W1997162244 cites W1993500767 @default.
- W1997162244 cites W1994225608 @default.
- W1997162244 cites W2006661331 @default.
- W1997162244 cites W2019402418 @default.
- W1997162244 cites W2028650153 @default.
- W1997162244 cites W2033756416 @default.
- W1997162244 cites W2035898869 @default.
- W1997162244 cites W2048582300 @default.
- W1997162244 cites W2062634740 @default.
- W1997162244 cites W2078724741 @default.
- W1997162244 cites W2462766199 @default.
- W1997162244 doi "https://doi.org/10.1104/pp.61.5.737" @default.
- W1997162244 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1091967" @default.
- W1997162244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16660375" @default.
- W1997162244 hasPublicationYear "1978" @default.
- W1997162244 type Work @default.
- W1997162244 sameAs 1997162244 @default.
- W1997162244 citedByCount "40" @default.
- W1997162244 countsByYear W19971622442012 @default.
- W1997162244 countsByYear W19971622442013 @default.
- W1997162244 countsByYear W19971622442014 @default.
- W1997162244 countsByYear W19971622442016 @default.
- W1997162244 countsByYear W19971622442022 @default.
- W1997162244 crossrefType "journal-article" @default.
- W1997162244 hasAuthorship W1997162244A5007103570 @default.
- W1997162244 hasAuthorship W1997162244A5060790939 @default.
- W1997162244 hasBestOaLocation W19971622441 @default.
- W1997162244 hasConcept C134121241 @default.
- W1997162244 hasConcept C134651460 @default.
- W1997162244 hasConcept C161790260 @default.
- W1997162244 hasConcept C163994747 @default.
- W1997162244 hasConcept C178790620 @default.
- W1997162244 hasConcept C181199279 @default.
- W1997162244 hasConcept C185592680 @default.
- W1997162244 hasConcept C191897082 @default.
- W1997162244 hasConcept C192562407 @default.
- W1997162244 hasConcept C193363816 @default.
- W1997162244 hasConcept C193557335 @default.
- W1997162244 hasConcept C197957613 @default.
- W1997162244 hasConcept C2779123261 @default.
- W1997162244 hasConcept C2781066024 @default.
- W1997162244 hasConcept C2781338088 @default.
- W1997162244 hasConcept C43617362 @default.
- W1997162244 hasConcept C55493867 @default.
- W1997162244 hasConcept C71240020 @default.
- W1997162244 hasConceptScore W1997162244C134121241 @default.
- W1997162244 hasConceptScore W1997162244C134651460 @default.
- W1997162244 hasConceptScore W1997162244C161790260 @default.
- W1997162244 hasConceptScore W1997162244C163994747 @default.
- W1997162244 hasConceptScore W1997162244C178790620 @default.
- W1997162244 hasConceptScore W1997162244C181199279 @default.
- W1997162244 hasConceptScore W1997162244C185592680 @default.
- W1997162244 hasConceptScore W1997162244C191897082 @default.
- W1997162244 hasConceptScore W1997162244C192562407 @default.
- W1997162244 hasConceptScore W1997162244C193363816 @default.
- W1997162244 hasConceptScore W1997162244C193557335 @default.
- W1997162244 hasConceptScore W1997162244C197957613 @default.
- W1997162244 hasConceptScore W1997162244C2779123261 @default.
- W1997162244 hasConceptScore W1997162244C2781066024 @default.
- W1997162244 hasConceptScore W1997162244C2781338088 @default.
- W1997162244 hasConceptScore W1997162244C43617362 @default.
- W1997162244 hasConceptScore W1997162244C55493867 @default.
- W1997162244 hasConceptScore W1997162244C71240020 @default.
- W1997162244 hasIssue "5" @default.
- W1997162244 hasLocation W19971622441 @default.
- W1997162244 hasLocation W19971622442 @default.
- W1997162244 hasLocation W19971622443 @default.
- W1997162244 hasOpenAccess W1997162244 @default.
- W1997162244 hasPrimaryLocation W19971622441 @default.
- W1997162244 hasRelatedWork W107896380 @default.
- W1997162244 hasRelatedWork W169735115 @default.
- W1997162244 hasRelatedWork W2072156423 @default.
- W1997162244 hasRelatedWork W2074263550 @default.
- W1997162244 hasRelatedWork W2094167934 @default.
- W1997162244 hasRelatedWork W2170874906 @default.
- W1997162244 hasRelatedWork W2806378807 @default.
- W1997162244 hasRelatedWork W2925386497 @default.
- W1997162244 hasRelatedWork W3951102 @default.
- W1997162244 hasRelatedWork W966738943 @default.
- W1997162244 hasVolume "61" @default.
- W1997162244 isParatext "false" @default.
- W1997162244 isRetracted "false" @default.
- W1997162244 magId "1997162244" @default.
- W1997162244 workType "article" @default.