Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997164023> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1997164023 endingPage "246" @default.
- W1997164023 startingPage "238" @default.
- W1997164023 abstract "The cytochemical detection of specific DNA sequences by hybridization with fluorochrome-labeled RNA and detection of the hybrids by fluorescence microscopy is described. RNAs complementary to the DNA of the kinetoplasts of Crithidia luciliae (an insect trypanosome) or to adenovirus-5 (Ad-5) DNA were labeled with the hydrazine derivative of tetramethylrhodamine isothiocyanate (TRITC). The specificity of the reactions between the complementary RNAs labeled both with 3H and tetramethylrhodamine was studied by cross-hybridization experiments using a model system in which the DNAs were bound to Sepharose beads. The extent of the reaction was measured by scintillation counting of the bead suspensions and quantitative fluorescence microscopy of individual Sepharose beads. The ability of the rhodamine-labeled cRNAs to hybridize and the absence of interference of the fluorochrome label with the specificity of the hybridization reaction was thus demonstrated. After cytochemical hybridization on microscopic preparations of C. luciliae cells the rhodamine-labeled kinetoplast cRNA stains only the kinetoplasts. No fluorescence was observed in the nuclei. After cytochemical hybridization of rhodamine-labeled Ad-5 cRNA with virus infected KB cells a distinct staining pattern in the nuclei was observed. No fluorescence was seen in uninfected cells, or after hybridization with heterologous rhodamine-labeled RNA. The possibilities and limitations of cytochemical hybridization with rhodamine-labeled RNA are discussed." @default.
- W1997164023 created "2016-06-24" @default.
- W1997164023 creator A5010503523 @default.
- W1997164023 creator A5049148156 @default.
- W1997164023 creator A5073969481 @default.
- W1997164023 date "1981-02-01" @default.
- W1997164023 modified "2023-09-27" @default.
- W1997164023 title "Cytochemical hybridization with fluorochrome-labeled RNA. II. Applications." @default.
- W1997164023 doi "https://doi.org/10.1177/29.2.6166654" @default.
- W1997164023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6166654" @default.
- W1997164023 hasPublicationYear "1981" @default.
- W1997164023 type Work @default.
- W1997164023 sameAs 1997164023 @default.
- W1997164023 citedByCount "51" @default.
- W1997164023 countsByYear W19971640232019 @default.
- W1997164023 crossrefType "journal-article" @default.
- W1997164023 hasAuthorship W1997164023A5010503523 @default.
- W1997164023 hasAuthorship W1997164023A5049148156 @default.
- W1997164023 hasAuthorship W1997164023A5073969481 @default.
- W1997164023 hasBestOaLocation W19971640231 @default.
- W1997164023 hasConcept C104317684 @default.
- W1997164023 hasConcept C105580179 @default.
- W1997164023 hasConcept C121332964 @default.
- W1997164023 hasConcept C130197536 @default.
- W1997164023 hasConcept C153911025 @default.
- W1997164023 hasConcept C181199279 @default.
- W1997164023 hasConcept C54355233 @default.
- W1997164023 hasConcept C54871578 @default.
- W1997164023 hasConcept C552990157 @default.
- W1997164023 hasConcept C55493867 @default.
- W1997164023 hasConcept C62520636 @default.
- W1997164023 hasConcept C67705224 @default.
- W1997164023 hasConcept C74864618 @default.
- W1997164023 hasConcept C81439078 @default.
- W1997164023 hasConcept C86803240 @default.
- W1997164023 hasConcept C91881484 @default.
- W1997164023 hasConcept C91999924 @default.
- W1997164023 hasConceptScore W1997164023C104317684 @default.
- W1997164023 hasConceptScore W1997164023C105580179 @default.
- W1997164023 hasConceptScore W1997164023C121332964 @default.
- W1997164023 hasConceptScore W1997164023C130197536 @default.
- W1997164023 hasConceptScore W1997164023C153911025 @default.
- W1997164023 hasConceptScore W1997164023C181199279 @default.
- W1997164023 hasConceptScore W1997164023C54355233 @default.
- W1997164023 hasConceptScore W1997164023C54871578 @default.
- W1997164023 hasConceptScore W1997164023C552990157 @default.
- W1997164023 hasConceptScore W1997164023C55493867 @default.
- W1997164023 hasConceptScore W1997164023C62520636 @default.
- W1997164023 hasConceptScore W1997164023C67705224 @default.
- W1997164023 hasConceptScore W1997164023C74864618 @default.
- W1997164023 hasConceptScore W1997164023C81439078 @default.
- W1997164023 hasConceptScore W1997164023C86803240 @default.
- W1997164023 hasConceptScore W1997164023C91881484 @default.
- W1997164023 hasConceptScore W1997164023C91999924 @default.
- W1997164023 hasIssue "2" @default.
- W1997164023 hasLocation W19971640231 @default.
- W1997164023 hasLocation W19971640232 @default.
- W1997164023 hasOpenAccess W1997164023 @default.
- W1997164023 hasPrimaryLocation W19971640231 @default.
- W1997164023 hasRelatedWork W13531407 @default.
- W1997164023 hasRelatedWork W2002473487 @default.
- W1997164023 hasRelatedWork W2061320859 @default.
- W1997164023 hasRelatedWork W2087312195 @default.
- W1997164023 hasRelatedWork W2298058195 @default.
- W1997164023 hasRelatedWork W2405162124 @default.
- W1997164023 hasRelatedWork W2406457652 @default.
- W1997164023 hasRelatedWork W2419348076 @default.
- W1997164023 hasRelatedWork W2422454227 @default.
- W1997164023 hasRelatedWork W657236858 @default.
- W1997164023 hasVolume "29" @default.
- W1997164023 isParatext "false" @default.
- W1997164023 isRetracted "false" @default.
- W1997164023 magId "1997164023" @default.
- W1997164023 workType "article" @default.