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- W1997164490 abstract "Background: Standard total parenteral nutrition (STD-TPN) may diminish host immunity as compared with enteral nutrition. Alanyl-Glutamine enriched TPN (Ala-Gln-TPN) has been suggested to restore immune defects caused by STD-TPN. This study is to investigate the effects of Ala-Gln-TPN on tumor necrosis factor (TNF) and interferon-γ (IFN-γ) production by splenocyte in vitro. Method: Rats were randomly divided into two groups as STD-TPN and Ala-Gln-TPN groups. They received isocaloric (199 kcal/kg/day) and isonitrogenous (Nitrogen: 2.2 gN/kg/day) nutrients for seven days, and were then sacrificed. Ala-Gln-TPN contained 30% (ww) of amino acid as glutamine. Twenty-four-hour culture of splenocytes (SC) with Gln at the concentration of 0, 500, and 1000 μM were performed in vitro. Supernatants were them collected for the measurement of TNF and IFN-γ. Results: IFN production by SC in vitro in the medium with 500 μM and 1000 μM of Gln were significantly greater in the Ala-Gln-TPN group than those in the STD-TPN group. However, TNF production by SC in vitro did not different significantly between the two groups. Conclusion: Ala-Gln-TPN may improve host immunity by enhancing IFN production from splenocytes." @default.
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- W1997164490 date "2000-06-01" @default.
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- W1997164490 title "Glutamine enriched total parenteral nutrition improves splenocyte interferon-γ production" @default.
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- W1997164490 doi "https://doi.org/10.1016/s0271-5317(00)00173-1" @default.
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