Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997265182> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1997265182 endingPage "241a" @default.
- W1997265182 startingPage "241a" @default.
- W1997265182 abstract "Persistent infections caused by antibiotic resistant microbes are a serious public health threat. Peptide antibiotics, which perturb the cell membrane, offer one promising solution. Critical characteristics of both natural and designed peptide antibiotics include the formation of ordered structures such as helices, and amphilicity. We are investigating the effects of electrostatics on the helicity of peptide antibiotic models that are composed primarily of the sterically hindered amino acid Aib, with Lys and Glu residues substituted at various positions in the helix. We report here results for the octameric peptides KK36 (Lys at positions 3 and 6, one 310-helical turn apart), EK36 (Glu and Lys at positions 3 and 6), EK45 (Glu and Lys adjacent in the center of the helix) and KK45 (two Lys adjacent in the center of the helix). NMR resonances were assigned using natural abundance 1H-13C HMBC and HSQC spectra. Distance constraints from 1H-1H ROESY and hydrogen-bonding information from amide temperature coefficients were used to calculate the three-dimensional structures of the peptides using Xplor-NIH. Global structural information was also obtained using CD spectroscopy. We find that KK36 and EK36 are 310-helical, with slightly different helical curvatures both in DMSO-d6 and in methanol-d3. CD spectra also indicate 310-helical structures in TFE and methanol, with a greater ratio of θ220/θ202 in TFE (∼0.4 versus ∼0.25). KK36 is less well structured in water versus organic solution, as evidenced by poor amide spectral dispersion. However, association of KK36 with lipid vesicles results in complete amide spectral dispersion, and an enhancement of the CD signal. The structures of all four peptides will be presented and interpreted in terms electrostatic effects on helicity in a variety of solvent systems." @default.
- W1997265182 created "2016-06-24" @default.
- W1997265182 creator A5001662145 @default.
- W1997265182 creator A5017771611 @default.
- W1997265182 creator A5036311124 @default.
- W1997265182 creator A5039013670 @default.
- W1997265182 creator A5065367306 @default.
- W1997265182 creator A5075077444 @default.
- W1997265182 creator A5089707111 @default.
- W1997265182 date "2013-01-01" @default.
- W1997265182 modified "2023-09-26" @default.
- W1997265182 title "Effects of Electrostatic Interactions on Helicity in Model Peptide Antibiotics using 2D NMR and CD Spectroscopies" @default.
- W1997265182 doi "https://doi.org/10.1016/j.bpj.2012.11.1358" @default.
- W1997265182 hasPublicationYear "2013" @default.
- W1997265182 type Work @default.
- W1997265182 sameAs 1997265182 @default.
- W1997265182 citedByCount "0" @default.
- W1997265182 crossrefType "journal-article" @default.
- W1997265182 hasAuthorship W1997265182A5001662145 @default.
- W1997265182 hasAuthorship W1997265182A5017771611 @default.
- W1997265182 hasAuthorship W1997265182A5036311124 @default.
- W1997265182 hasAuthorship W1997265182A5039013670 @default.
- W1997265182 hasAuthorship W1997265182A5065367306 @default.
- W1997265182 hasAuthorship W1997265182A5075077444 @default.
- W1997265182 hasAuthorship W1997265182A5089707111 @default.
- W1997265182 hasBestOaLocation W19972651821 @default.
- W1997265182 hasConcept C109214941 @default.
- W1997265182 hasConcept C112887158 @default.
- W1997265182 hasConcept C121332964 @default.
- W1997265182 hasConcept C178790620 @default.
- W1997265182 hasConcept C185592680 @default.
- W1997265182 hasConcept C18903297 @default.
- W1997265182 hasConcept C193125475 @default.
- W1997265182 hasConcept C201194858 @default.
- W1997265182 hasConcept C2775859700 @default.
- W1997265182 hasConcept C2778439535 @default.
- W1997265182 hasConcept C2778530040 @default.
- W1997265182 hasConcept C2779281246 @default.
- W1997265182 hasConcept C2779965526 @default.
- W1997265182 hasConcept C32909587 @default.
- W1997265182 hasConcept C515207424 @default.
- W1997265182 hasConcept C55493867 @default.
- W1997265182 hasConcept C62614982 @default.
- W1997265182 hasConcept C66974803 @default.
- W1997265182 hasConcept C71240020 @default.
- W1997265182 hasConcept C8010536 @default.
- W1997265182 hasConcept C86803240 @default.
- W1997265182 hasConceptScore W1997265182C109214941 @default.
- W1997265182 hasConceptScore W1997265182C112887158 @default.
- W1997265182 hasConceptScore W1997265182C121332964 @default.
- W1997265182 hasConceptScore W1997265182C178790620 @default.
- W1997265182 hasConceptScore W1997265182C185592680 @default.
- W1997265182 hasConceptScore W1997265182C18903297 @default.
- W1997265182 hasConceptScore W1997265182C193125475 @default.
- W1997265182 hasConceptScore W1997265182C201194858 @default.
- W1997265182 hasConceptScore W1997265182C2775859700 @default.
- W1997265182 hasConceptScore W1997265182C2778439535 @default.
- W1997265182 hasConceptScore W1997265182C2778530040 @default.
- W1997265182 hasConceptScore W1997265182C2779281246 @default.
- W1997265182 hasConceptScore W1997265182C2779965526 @default.
- W1997265182 hasConceptScore W1997265182C32909587 @default.
- W1997265182 hasConceptScore W1997265182C515207424 @default.
- W1997265182 hasConceptScore W1997265182C55493867 @default.
- W1997265182 hasConceptScore W1997265182C62614982 @default.
- W1997265182 hasConceptScore W1997265182C66974803 @default.
- W1997265182 hasConceptScore W1997265182C71240020 @default.
- W1997265182 hasConceptScore W1997265182C8010536 @default.
- W1997265182 hasConceptScore W1997265182C86803240 @default.
- W1997265182 hasIssue "2" @default.
- W1997265182 hasLocation W19972651821 @default.
- W1997265182 hasOpenAccess W1997265182 @default.
- W1997265182 hasPrimaryLocation W19972651821 @default.
- W1997265182 hasRelatedWork W2006264800 @default.
- W1997265182 hasRelatedWork W2014232222 @default.
- W1997265182 hasRelatedWork W2021603413 @default.
- W1997265182 hasRelatedWork W2021942294 @default.
- W1997265182 hasRelatedWork W2027886505 @default.
- W1997265182 hasRelatedWork W2039214627 @default.
- W1997265182 hasRelatedWork W2041829915 @default.
- W1997265182 hasRelatedWork W2066915015 @default.
- W1997265182 hasRelatedWork W4230612908 @default.
- W1997265182 hasRelatedWork W4320801379 @default.
- W1997265182 hasVolume "104" @default.
- W1997265182 isParatext "false" @default.
- W1997265182 isRetracted "false" @default.
- W1997265182 magId "1997265182" @default.
- W1997265182 workType "article" @default.