FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997334561> ?p ?o ?g. }

• W1997334561 endingPage "23" @default.
• W1997334561 startingPage "13" @default.
• W1997334561 abstract "17β-Hydroxysteroid dehydrogenase, which catalyzes oxidoreduction between androstenedione and testosterone, has been solubilized by sonication from porcine testicular microsomal membrane for the first time. From the solubilized enzyme preparation, the dehydrogenase activity was precipitated by between 40 and 75% saturation of ammonium sulfate. After the precipitate was dissolved, the enzyme solution was applied to a Sephadex G-100 column and the dehydrogenase activity emerged at 1.80 void volumes. For further purification, it was subjected to DEAE-cellulose column chromatography; by eluting with a linear gradient of increasing phosphate concentration, the enzyme activity between 15 and 20 mM phosphate. Finally, when the partially purified enzyme preparation was applied to a Bio-Gel P-100 column, the dehydrogenase activity emerged at 1.46 void volumes. The final enzyme preparation was purified 1880-fold in specific activity compared to the microsomal fraction. The purified enzyme preparation approached apparent homogeneity, checked by discontinuous polyacrylamide gel electrophoresis at pH 8.3 and by thin-layer gel chromatography. The final preparation was able to produce 67.7 nmol testosterone from androstenedione per min per mg protein at 37°C in the presence of NADPH, and could be stored at–20°C for 6 months with no significant loss of the enzyme activity. The molecular weight and molecular radius of the 17β-hydroxysteroid dehydrogenase was estimated to be about 35500 and 2.37 nm, respectively, from the results of the gel filtration chromatography. In the phosphate buffer pH 7.4 maximal rate of reduction of androstenedione by the enzyme was observed at 50°C, and Q10 for the enzyme was 3.7, while the activation energy was 19500 cal. The rate of reduction of androstenedione at 37.5°C was about a half of the maximal one at 50°C in the phosphate buffer, whereas the reduction rate at 37°C was higher than the one at 50°C in the citric acid-Na2HPO4 buffer pH 7.3. The enzyme for androstenedione showed a pH optimum between 6.5 and 7.5 in the presence of NADPH at 37°C but the optimal pH of the enzyme was shifted to 8.5 by raising the incubation temperature to 50°C. Estrone was the acceptor of the hydrogen released from NADPH by the 17β-hydroxysteroid dehydrogenase and dehydroepiandrosterone served a similar purpose for androstenedione. The oxidation of testosterone by the enzyme in the presence of NADP+ proceeded at the same rate as the reduction of androstenedione. The purified enzyme preparation was devoid of any alcohol or 3-hydroxyhexobarbital dehydrogenase activities or other enzymes related to testicular steroidogenesis." @default.
• W1997334561 created "2016-06-24" @default.
• W1997334561 creator A5038839757 @default.
• W1997334561 creator A5077665206 @default.
• W1997334561 date "1974-05-01" @default.
• W1997334561 modified "2023-09-27" @default.
• W1997334561 title "Purification and Properties of NADP-Dependent 17beta-Hydroxysteroid Dehydrogenase Solubilized from Porcine-Testicular Microsomal Fraction" @default.
• W1997334561 cites W1484478694 @default.
• W1997334561 cites W1487108495 @default.
• W1997334561 cites W1507638955 @default.
• W1997334561 cites W1525263001 @default.
• W1997334561 cites W1528655921 @default.
• W1997334561 cites W1535735436 @default.
• W1997334561 cites W1554289400 @default.
• W1997334561 cites W1601970533 @default.
• W1997334561 cites W1611482599 @default.
• W1997334561 cites W188830599 @default.
• W1997334561 cites W1938912494 @default.
• W1997334561 cites W1967600222 @default.
• W1997334561 cites W1982950734 @default.
• W1997334561 cites W1992002350 @default.
• W1997334561 cites W1997248232 @default.
• W1997334561 cites W1998089380 @default.
• W1997334561 cites W2012177357 @default.
• W1997334561 cites W2016112382 @default.
• W1997334561 cites W2020619618 @default.
• W1997334561 cites W2022665357 @default.
• W1997334561 cites W2033259765 @default.
• W1997334561 cites W2039657724 @default.
• W1997334561 cites W2040116655 @default.
• W1997334561 cites W2043762391 @default.
• W1997334561 cites W2045036147 @default.
• W1997334561 cites W2045728044 @default.
• W1997334561 cites W2047002543 @default.
• W1997334561 cites W2077945132 @default.
• W1997334561 cites W2081640493 @default.
• W1997334561 cites W2083945806 @default.
• W1997334561 cites W2085429459 @default.
• W1997334561 cites W2111301002 @default.
• W1997334561 cites W2265510311 @default.
• W1997334561 cites W2409874733 @default.
• W1997334561 cites W33806248 @default.
• W1997334561 doi "https://doi.org/10.1111/j.1432-1033.1974.tb03453.x" @default.
• W1997334561 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4153029" @default.
• W1997334561 hasPublicationYear "1974" @default.
• W1997334561 type Work @default.
• W1997334561 sameAs 1997334561 @default.
• W1997334561 citedByCount "56" @default.
• W1997334561 crossrefType "journal-article" @default.
• W1997334561 hasAuthorship W1997334561A5038839757 @default.
• W1997334561 hasAuthorship W1997334561A5077665206 @default.
• W1997334561 hasConcept C163994747 @default.
• W1997334561 hasConcept C181199279 @default.
• W1997334561 hasConcept C185592680 @default.
• W1997334561 hasConcept C193363816 @default.
• W1997334561 hasConcept C2776317432 @default.
• W1997334561 hasConcept C2776683971 @default.
• W1997334561 hasConcept C2777911890 @default.
• W1997334561 hasConcept C2781067048 @default.
• W1997334561 hasConcept C28212737 @default.
• W1997334561 hasConcept C43617362 @default.
• W1997334561 hasConcept C55493867 @default.
• W1997334561 hasConcept C71315377 @default.
• W1997334561 hasConceptScore W1997334561C163994747 @default.
• W1997334561 hasConceptScore W1997334561C181199279 @default.
• W1997334561 hasConceptScore W1997334561C185592680 @default.
• W1997334561 hasConceptScore W1997334561C193363816 @default.
• W1997334561 hasConceptScore W1997334561C2776317432 @default.
• W1997334561 hasConceptScore W1997334561C2776683971 @default.
• W1997334561 hasConceptScore W1997334561C2777911890 @default.
• W1997334561 hasConceptScore W1997334561C2781067048 @default.
• W1997334561 hasConceptScore W1997334561C28212737 @default.
• W1997334561 hasConceptScore W1997334561C43617362 @default.
• W1997334561 hasConceptScore W1997334561C55493867 @default.
• W1997334561 hasConceptScore W1997334561C71315377 @default.
• W1997334561 hasIssue "1" @default.
• W1997334561 hasLocation W19973345611 @default.
• W1997334561 hasLocation W19973345612 @default.
• W1997334561 hasOpenAccess W1997334561 @default.
• W1997334561 hasPrimaryLocation W19973345611 @default.
• W1997334561 hasRelatedWork W1649084527 @default.
• W1997334561 hasRelatedWork W1923316703 @default.
• W1997334561 hasRelatedWork W2019830471 @default.
• W1997334561 hasRelatedWork W2024817429 @default.
• W1997334561 hasRelatedWork W2078909520 @default.
• W1997334561 hasRelatedWork W2138791828 @default.
• W1997334561 hasRelatedWork W2367026271 @default.
• W1997334561 hasRelatedWork W2414651548 @default.
• W1997334561 hasRelatedWork W2434264816 @default.
• W1997334561 hasRelatedWork W868588551 @default.
• W1997334561 hasVolume "44" @default.
• W1997334561 isParatext "false" @default.
• W1997334561 isRetracted "false" @default.
• W1997334561 magId "1997334561" @default.
• W1997334561 workType "article" @default.