Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997394955> ?p ?o ?g. }
- W1997394955 endingPage "2032" @default.
- W1997394955 startingPage "2028" @default.
- W1997394955 abstract "Different preparations of human rhinovirus serotype 2 (HRV2), a common cold virus, were analyzed by capillary zone electrophoresis (CZE) in untreated fused-silica capillaries using borate buffer (100 mmol/L, pH 8.3) and sodium dodecyl sulfate (10 mmol/L) as additive to prevent wall adsorption. The electropherograms showed one major peak at 205- and 254-nm detection wavelengths. The identity of the peak as originating from native virus was confirmed by several indirect methods. Heating to 56 °C is known to lead to release of the genomic RNA from the viral capsid; this treatment resulted in the disappearance of the major peak and the emergence of a new predominant peak that was identified as RNA by enzymatic digestion. As expected, RNase treatment of the unheated sample remained without effect as the viral genome is inaccessible in the native viral shell. A monoclonal, virus-aggregating antibody was used for immunodepletion of native virus; again, the major peak disappeared upon removal of viral aggregates by centrifugation prior to CZE analysis. In combination, these results allowed for the unambiguous identification of the main peak as native HRV2 and of the minor peaks as contaminants present in various amounts in the different viral preparations. It is demonstrated that CZE allows for an extremely easy and rapid assessment of conformational state and purity of virions in a given viral preparation." @default.
- W1997394955 created "2016-06-24" @default.
- W1997394955 creator A5002011879 @default.
- W1997394955 creator A5029312136 @default.
- W1997394955 creator A5044972383 @default.
- W1997394955 creator A5047255401 @default.
- W1997394955 date "1999-04-09" @default.
- W1997394955 modified "2023-10-10" @default.
- W1997394955 title "Analysis of Common Cold Virus (Human Rhinovirus Serotype 2) by Capillary Zone Electrophoresis: The Problem of Peak Identification" @default.
- W1997394955 cites W194037594 @default.
- W1997394955 cites W1970657345 @default.
- W1997394955 cites W1970806557 @default.
- W1997394955 cites W1977314808 @default.
- W1997394955 cites W1989148327 @default.
- W1997394955 cites W1996432946 @default.
- W1997394955 cites W2017759047 @default.
- W1997394955 cites W2022111622 @default.
- W1997394955 cites W2025737481 @default.
- W1997394955 cites W2027386772 @default.
- W1997394955 cites W2048305624 @default.
- W1997394955 cites W2053075712 @default.
- W1997394955 cites W2053235616 @default.
- W1997394955 cites W2056096329 @default.
- W1997394955 cites W2077400936 @default.
- W1997394955 cites W2080199866 @default.
- W1997394955 cites W2155817823 @default.
- W1997394955 cites W2168952850 @default.
- W1997394955 cites W4239647016 @default.
- W1997394955 doi "https://doi.org/10.1021/ac981324x" @default.
- W1997394955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10361502" @default.
- W1997394955 hasPublicationYear "1999" @default.
- W1997394955 type Work @default.
- W1997394955 sameAs 1997394955 @default.
- W1997394955 citedByCount "68" @default.
- W1997394955 countsByYear W19973949552012 @default.
- W1997394955 countsByYear W19973949552013 @default.
- W1997394955 countsByYear W19973949552014 @default.
- W1997394955 countsByYear W19973949552016 @default.
- W1997394955 countsByYear W19973949552017 @default.
- W1997394955 countsByYear W19973949552018 @default.
- W1997394955 countsByYear W19973949552019 @default.
- W1997394955 countsByYear W19973949552021 @default.
- W1997394955 countsByYear W19973949552023 @default.
- W1997394955 crossrefType "journal-article" @default.
- W1997394955 hasAuthorship W1997394955A5002011879 @default.
- W1997394955 hasAuthorship W1997394955A5029312136 @default.
- W1997394955 hasAuthorship W1997394955A5044972383 @default.
- W1997394955 hasAuthorship W1997394955A5047255401 @default.
- W1997394955 hasConcept C104317684 @default.
- W1997394955 hasConcept C10879258 @default.
- W1997394955 hasConcept C153911025 @default.
- W1997394955 hasConcept C159047783 @default.
- W1997394955 hasConcept C185592680 @default.
- W1997394955 hasConcept C19472624 @default.
- W1997394955 hasConcept C202878990 @default.
- W1997394955 hasConcept C2522874641 @default.
- W1997394955 hasConcept C2778913249 @default.
- W1997394955 hasConcept C2900643 @default.
- W1997394955 hasConcept C40250595 @default.
- W1997394955 hasConcept C43617362 @default.
- W1997394955 hasConcept C55493867 @default.
- W1997394955 hasConcept C67705224 @default.
- W1997394955 hasConcept C7623868 @default.
- W1997394955 hasConcept C86803240 @default.
- W1997394955 hasConceptScore W1997394955C104317684 @default.
- W1997394955 hasConceptScore W1997394955C10879258 @default.
- W1997394955 hasConceptScore W1997394955C153911025 @default.
- W1997394955 hasConceptScore W1997394955C159047783 @default.
- W1997394955 hasConceptScore W1997394955C185592680 @default.
- W1997394955 hasConceptScore W1997394955C19472624 @default.
- W1997394955 hasConceptScore W1997394955C202878990 @default.
- W1997394955 hasConceptScore W1997394955C2522874641 @default.
- W1997394955 hasConceptScore W1997394955C2778913249 @default.
- W1997394955 hasConceptScore W1997394955C2900643 @default.
- W1997394955 hasConceptScore W1997394955C40250595 @default.
- W1997394955 hasConceptScore W1997394955C43617362 @default.
- W1997394955 hasConceptScore W1997394955C55493867 @default.
- W1997394955 hasConceptScore W1997394955C67705224 @default.
- W1997394955 hasConceptScore W1997394955C7623868 @default.
- W1997394955 hasConceptScore W1997394955C86803240 @default.
- W1997394955 hasIssue "10" @default.
- W1997394955 hasLocation W19973949551 @default.
- W1997394955 hasLocation W19973949552 @default.
- W1997394955 hasOpenAccess W1997394955 @default.
- W1997394955 hasPrimaryLocation W19973949551 @default.
- W1997394955 hasRelatedWork W1528827813 @default.
- W1997394955 hasRelatedWork W1966225535 @default.
- W1997394955 hasRelatedWork W1969271865 @default.
- W1997394955 hasRelatedWork W1969386671 @default.
- W1997394955 hasRelatedWork W1998962854 @default.
- W1997394955 hasRelatedWork W2013704334 @default.
- W1997394955 hasRelatedWork W2077855486 @default.
- W1997394955 hasRelatedWork W2086792757 @default.
- W1997394955 hasRelatedWork W2090258789 @default.
- W1997394955 hasRelatedWork W2127511777 @default.
- W1997394955 hasVolume "71" @default.
- W1997394955 isParatext "false" @default.
- W1997394955 isRetracted "false" @default.