FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997403931> ?p ?o ?g. }

• W1997403931 endingPage "815" @default.
• W1997403931 startingPage "805" @default.
• W1997403931 abstract "The purpose of this study was to determine if chondrocytes cultured through several subcultures at very low plating density would produce new cartilage matrix after being reimplanted in vivo with or without a hydrogel polymer scaffold. Chondrocytes were initially plated in low-density monolayer culture, grown to confluence, and passaged four times. After each passage cells were suspended in purified porcine fibrinogen and injected into the subcutaneous space of nude mice while simultaneously polymerizing with thrombin to reach a final concentration of 40 million cells/cc. Controls were made by injecting fresh, uncultured cells with fibrin polymer and by injecting the cultured cells in saline (without polymer). All samples were harvested at 6 weeks. When injected in polymer, both fresh cells and cells that had undergone only one passage in culture produced cartilaginous nodules. Cultured cells did not produce cartilage, regardless of length of time spent in culture, when injected without polymer. Cartilage was also not recovered from samples with cells kept in culture for longer than one passage, even when provided with a polymer matrix. All samples harvested were subjected to histological analysis and assayed for total DNA, glycosaminoglycan (GAG), and type II collagen. There was histological evidence of cartilage in the groups that used fresh cells and cultured cells suspended in fibrin polymer that only underwent one passage. No other group contained areas that would be consistent with cartilage histologically. All experimental samples had a higher percent of DNA than native swine cartilage, and there was no statistical difference between the DNA content of the groups containing cultured or fresh cells in fibrin polymer. Whereas the GAG content of native cartilage was 8.39% of dry weight and fresh cells in fibrin polymer was 12.85%, cultured cells in fibrin polymer never exceded the 2.48% noted from first passage cells. In conclusion, this study demonstrates that porcine chondrocytes that have been cultured in monolayer for one passage will produce cartilage in vivo when suspended in fibrin polymer." @default.
• W1997403931 created "2016-06-24" @default.
• W1997403931 creator A5017596802 @default.
• W1997403931 creator A5017960330 @default.
• W1997403931 creator A5027685823 @default.
• W1997403931 creator A5034351333 @default.
• W1997403931 creator A5048238441 @default.
• W1997403931 creator A5058513985 @default.
• W1997403931 creator A5072839041 @default.
• W1997403931 date "2001-12-01" @default.
• W1997403931 modified "2023-10-18" @default.
• W1997403931 title "Cultured Chondrocytes Produce Injectable Tissue-Engineered Cartilage in Hydrogel Polymer" @default.
• W1997403931 cites W1967269017 @default.
• W1997403931 cites W1978035620 @default.
• W1997403931 cites W1978478023 @default.
• W1997403931 cites W1988592246 @default.
• W1997403931 cites W1990796508 @default.
• W1997403931 cites W2003731025 @default.
• W1997403931 cites W2012544592 @default.
• W1997403931 cites W2028981877 @default.
• W1997403931 cites W2047943571 @default.
• W1997403931 cites W2050100752 @default.
• W1997403931 cites W2050273631 @default.
• W1997403931 cites W2051733665 @default.
• W1997403931 cites W2082687207 @default.
• W1997403931 cites W2084479143 @default.
• W1997403931 cites W2091464294 @default.
• W1997403931 cites W2093066616 @default.
• W1997403931 cites W2187684329 @default.
• W1997403931 doi "https://doi.org/10.1089/107632701753337744" @default.
• W1997403931 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11749736" @default.
• W1997403931 hasPublicationYear "2001" @default.
• W1997403931 type Work @default.
• W1997403931 sameAs 1997403931 @default.
• W1997403931 citedByCount "108" @default.
• W1997403931 countsByYear W19974039312012 @default.
• W1997403931 countsByYear W19974039312013 @default.
• W1997403931 countsByYear W19974039312014 @default.
• W1997403931 countsByYear W19974039312015 @default.
• W1997403931 countsByYear W19974039312016 @default.
• W1997403931 countsByYear W19974039312017 @default.
• W1997403931 countsByYear W19974039312018 @default.
• W1997403931 countsByYear W19974039312019 @default.
• W1997403931 countsByYear W19974039312020 @default.
• W1997403931 countsByYear W19974039312021 @default.
• W1997403931 countsByYear W19974039312022 @default.
• W1997403931 countsByYear W19974039312023 @default.
• W1997403931 crossrefType "journal-article" @default.
• W1997403931 hasAuthorship W1997403931A5017596802 @default.
• W1997403931 hasAuthorship W1997403931A5017960330 @default.
• W1997403931 hasAuthorship W1997403931A5027685823 @default.
• W1997403931 hasAuthorship W1997403931A5034351333 @default.
• W1997403931 hasAuthorship W1997403931A5048238441 @default.
• W1997403931 hasAuthorship W1997403931A5058513985 @default.
• W1997403931 hasAuthorship W1997403931A5072839041 @default.
• W1997403931 hasConcept C105702510 @default.
• W1997403931 hasConcept C106487976 @default.
• W1997403931 hasConcept C136229726 @default.
• W1997403931 hasConcept C153074725 @default.
• W1997403931 hasConcept C153911025 @default.
• W1997403931 hasConcept C185592680 @default.
• W1997403931 hasConcept C202751555 @default.
• W1997403931 hasConcept C203014093 @default.
• W1997403931 hasConcept C2780550940 @default.
• W1997403931 hasConcept C43617362 @default.
• W1997403931 hasConcept C49892992 @default.
• W1997403931 hasConcept C54173615 @default.
• W1997403931 hasConcept C55493867 @default.
• W1997403931 hasConcept C71924100 @default.
• W1997403931 hasConcept C86803240 @default.
• W1997403931 hasConcept C90703135 @default.
• W1997403931 hasConceptScore W1997403931C105702510 @default.
• W1997403931 hasConceptScore W1997403931C106487976 @default.
• W1997403931 hasConceptScore W1997403931C136229726 @default.
• W1997403931 hasConceptScore W1997403931C153074725 @default.
• W1997403931 hasConceptScore W1997403931C153911025 @default.
• W1997403931 hasConceptScore W1997403931C185592680 @default.
• W1997403931 hasConceptScore W1997403931C202751555 @default.
• W1997403931 hasConceptScore W1997403931C203014093 @default.
• W1997403931 hasConceptScore W1997403931C2780550940 @default.
• W1997403931 hasConceptScore W1997403931C43617362 @default.
• W1997403931 hasConceptScore W1997403931C49892992 @default.
• W1997403931 hasConceptScore W1997403931C54173615 @default.
• W1997403931 hasConceptScore W1997403931C55493867 @default.
• W1997403931 hasConceptScore W1997403931C71924100 @default.
• W1997403931 hasConceptScore W1997403931C86803240 @default.
• W1997403931 hasConceptScore W1997403931C90703135 @default.
• W1997403931 hasIssue "6" @default.
• W1997403931 hasLocation W19974039311 @default.
• W1997403931 hasLocation W19974039312 @default.
• W1997403931 hasOpenAccess W1997403931 @default.
• W1997403931 hasPrimaryLocation W19974039311 @default.
• W1997403931 hasRelatedWork W1996932443 @default.
• W1997403931 hasRelatedWork W2012171708 @default.
• W1997403931 hasRelatedWork W2032255522 @default.
• W1997403931 hasRelatedWork W2042559978 @default.
• W1997403931 hasRelatedWork W2081519775 @default.
• W1997403931 hasRelatedWork W2155643123 @default.

• next