Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997411497> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1997411497 endingPage "130" @default.
- W1997411497 startingPage "121" @default.
- W1997411497 abstract "We synthesized a novel ligand [4,5-3H-Leu9]-Neurokinin A (3H-NKA, S.A 117–144 Ci/mmol), and evaluated its binding to hamster urinary bladder membranes (HUBM). The ligand bound to HUBM in a highly-specific (94 ± 4%) and protein-dependent manner. Binding was rapid (k1 = 0.037 nM−1∗min−1) and saturable (Bmax = 1210 ± 177 fmol/mg protein), to a single population of high-affinity sites (KD = 2.41 ± 0.15 nM, nH = 0.99 ± 0.02). Binding was inhibited by non-hydrolyzable GTP analogs. Competition experiments with HUBM demonstrated the following rank order of potency: NKA > Kassinin > [β-Ala8]-NKA(4–10) > [Nle10]-NKA(4–10) = Eledoisin = NKB > Physaelamin > Substance P. The selective NK-1 and NK-3 ligands, [Sar9-Met (O2)11]-SP, (±) CP96,345 and Senktide respectively, did not inhibit binding at 10 μM, whereas, the selective NK-2 antagonists: (±) SR-48,968 ⪢ L-659,877 > R396 ⪢ MEN-10,207 > MEN-10,376, inhibited binding in a competitive manner. In contrast, the low specific binding (<30%) detected in guinea pig lung membranes, was not inhibited by selective NK-2 ligands. Over 30 ligands (0.1–10 μM) from other receptor classes, were not inhibitory. The data suggest that this new ligand binds with high-affinity and selectivity to homogeneous population of NK-2 receptors on HUBM but not on lung membranes, and is a suitable ligand to study NK-2 receptors." @default.
- W1997411497 created "2016-06-24" @default.
- W1997411497 creator A5002808336 @default.
- W1997411497 creator A5019181764 @default.
- W1997411497 creator A5056943098 @default.
- W1997411497 date "1992-10-01" @default.
- W1997411497 modified "2023-10-14" @default.
- W1997411497 title "Pharmacologic characterization of the novel ligand [4,5-3H-Leu9]Neurokinin-A binding to NK-2 receptors on hamster urinary bladder membranes" @default.
- W1997411497 cites W1510358804 @default.
- W1997411497 cites W1979951815 @default.
- W1997411497 cites W1983636660 @default.
- W1997411497 cites W1990070700 @default.
- W1997411497 cites W1990880840 @default.
- W1997411497 cites W2006156987 @default.
- W1997411497 cites W2026126501 @default.
- W1997411497 cites W2032935189 @default.
- W1997411497 cites W2051887203 @default.
- W1997411497 cites W2059900452 @default.
- W1997411497 cites W2066036478 @default.
- W1997411497 cites W2074631079 @default.
- W1997411497 cites W2079052515 @default.
- W1997411497 cites W2091303128 @default.
- W1997411497 cites W2103547359 @default.
- W1997411497 cites W2107858856 @default.
- W1997411497 cites W4247823936 @default.
- W1997411497 doi "https://doi.org/10.1016/0143-4179(92)90089-f" @default.
- W1997411497 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1333574" @default.
- W1997411497 hasPublicationYear "1992" @default.
- W1997411497 type Work @default.
- W1997411497 sameAs 1997411497 @default.
- W1997411497 citedByCount "9" @default.
- W1997411497 crossrefType "journal-article" @default.
- W1997411497 hasAuthorship W1997411497A5002808336 @default.
- W1997411497 hasAuthorship W1997411497A5019181764 @default.
- W1997411497 hasAuthorship W1997411497A5056943098 @default.
- W1997411497 hasConcept C107824862 @default.
- W1997411497 hasConcept C116569031 @default.
- W1997411497 hasConcept C118303440 @default.
- W1997411497 hasConcept C126322002 @default.
- W1997411497 hasConcept C134018914 @default.
- W1997411497 hasConcept C170493617 @default.
- W1997411497 hasConcept C185592680 @default.
- W1997411497 hasConcept C2776533618 @default.
- W1997411497 hasConcept C2777056410 @default.
- W1997411497 hasConcept C2778575167 @default.
- W1997411497 hasConcept C2779874015 @default.
- W1997411497 hasConcept C2781359426 @default.
- W1997411497 hasConcept C2908647359 @default.
- W1997411497 hasConcept C55493867 @default.
- W1997411497 hasConcept C55938493 @default.
- W1997411497 hasConcept C67847695 @default.
- W1997411497 hasConcept C71924100 @default.
- W1997411497 hasConcept C86803240 @default.
- W1997411497 hasConcept C99454951 @default.
- W1997411497 hasConceptScore W1997411497C107824862 @default.
- W1997411497 hasConceptScore W1997411497C116569031 @default.
- W1997411497 hasConceptScore W1997411497C118303440 @default.
- W1997411497 hasConceptScore W1997411497C126322002 @default.
- W1997411497 hasConceptScore W1997411497C134018914 @default.
- W1997411497 hasConceptScore W1997411497C170493617 @default.
- W1997411497 hasConceptScore W1997411497C185592680 @default.
- W1997411497 hasConceptScore W1997411497C2776533618 @default.
- W1997411497 hasConceptScore W1997411497C2777056410 @default.
- W1997411497 hasConceptScore W1997411497C2778575167 @default.
- W1997411497 hasConceptScore W1997411497C2779874015 @default.
- W1997411497 hasConceptScore W1997411497C2781359426 @default.
- W1997411497 hasConceptScore W1997411497C2908647359 @default.
- W1997411497 hasConceptScore W1997411497C55493867 @default.
- W1997411497 hasConceptScore W1997411497C55938493 @default.
- W1997411497 hasConceptScore W1997411497C67847695 @default.
- W1997411497 hasConceptScore W1997411497C71924100 @default.
- W1997411497 hasConceptScore W1997411497C86803240 @default.
- W1997411497 hasConceptScore W1997411497C99454951 @default.
- W1997411497 hasIssue "2" @default.
- W1997411497 hasLocation W19974114971 @default.
- W1997411497 hasLocation W19974114972 @default.
- W1997411497 hasOpenAccess W1997411497 @default.
- W1997411497 hasPrimaryLocation W19974114971 @default.
- W1997411497 hasRelatedWork W1997067647 @default.
- W1997411497 hasRelatedWork W2037485320 @default.
- W1997411497 hasRelatedWork W2054381443 @default.
- W1997411497 hasRelatedWork W2064272350 @default.
- W1997411497 hasRelatedWork W2076838713 @default.
- W1997411497 hasRelatedWork W2087152274 @default.
- W1997411497 hasRelatedWork W2134139741 @default.
- W1997411497 hasRelatedWork W2471848544 @default.
- W1997411497 hasRelatedWork W4256723616 @default.
- W1997411497 hasRelatedWork W48988146 @default.
- W1997411497 hasVolume "23" @default.
- W1997411497 isParatext "false" @default.
- W1997411497 isRetracted "false" @default.
- W1997411497 magId "1997411497" @default.
- W1997411497 workType "article" @default.