Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997455905> ?p ?o ?g. }
- W1997455905 endingPage "1224" @default.
- W1997455905 startingPage "1211" @default.
- W1997455905 abstract "We have used a novel method to activate the DNA damage S-phase checkpoint response in Saccharomyces cerevisiae to slow lagging-strand DNA replication by exposing cells expressing a drug-sensitive DNA polymerase δ (L612M-DNA pol δ) to the inhibitory drug phosphonoacetic acid (PAA). PAA-treated pol3-L612M cells arrest as large-budded cells with a single nucleus in the bud neck. This arrest requires all of the components of the S-phase DNA damage checkpoint: Mec1, Rad9, the DNA damage clamp Ddc1-Rad17-Mec3, and the Rad24-dependent clamp loader, but does not depend on Mrc1, which acts as the signaling adapter for the replication checkpoint. In addition to the above components, a fully functional mismatch repair system, including Exo1, is required to activate the S-phase damage checkpoint and for cells to survive drug exposure. We propose that mismatch repair activity produces persisting single-stranded DNA gaps in PAA-treated pol3-L612M cells that are required to increase DNA damage above the threshold needed for checkpoint activation. Our studies have important implications for understanding how cells avoid inappropriate checkpoint activation because of normal discontinuities in lagging-strand replication and identify a role for mismatch repair in checkpoint activation that is needed to maintain genome integrity." @default.
- W1997455905 created "2016-06-24" @default.
- W1997455905 creator A5023165801 @default.
- W1997455905 creator A5031007485 @default.
- W1997455905 creator A5036797836 @default.
- W1997455905 creator A5037768394 @default.
- W1997455905 creator A5052529961 @default.
- W1997455905 creator A5058543639 @default.
- W1997455905 creator A5062169677 @default.
- W1997455905 creator A5062797658 @default.
- W1997455905 date "2011-12-01" @default.
- W1997455905 modified "2023-10-14" @default.
- W1997455905 title "Drug-Sensitive DNA Polymerase δ Reveals a Role for Mismatch Repair in Checkpoint Activation in Yeast" @default.
- W1997455905 cites W1480404031 @default.
- W1997455905 cites W1564916342 @default.
- W1997455905 cites W1636161271 @default.
- W1997455905 cites W1902420624 @default.
- W1997455905 cites W1918680390 @default.
- W1997455905 cites W1963876890 @default.
- W1997455905 cites W1968895302 @default.
- W1997455905 cites W1974425687 @default.
- W1997455905 cites W1991539934 @default.
- W1997455905 cites W1998205845 @default.
- W1997455905 cites W2005353346 @default.
- W1997455905 cites W2011917032 @default.
- W1997455905 cites W2019394964 @default.
- W1997455905 cites W2021347463 @default.
- W1997455905 cites W2029361378 @default.
- W1997455905 cites W2037898576 @default.
- W1997455905 cites W2043895957 @default.
- W1997455905 cites W2052442016 @default.
- W1997455905 cites W2055667487 @default.
- W1997455905 cites W2059184834 @default.
- W1997455905 cites W2062056341 @default.
- W1997455905 cites W2067755986 @default.
- W1997455905 cites W2077640472 @default.
- W1997455905 cites W2078242581 @default.
- W1997455905 cites W2078957361 @default.
- W1997455905 cites W2083753924 @default.
- W1997455905 cites W2084147994 @default.
- W1997455905 cites W2089068108 @default.
- W1997455905 cites W2089468535 @default.
- W1997455905 cites W2089594949 @default.
- W1997455905 cites W2092282016 @default.
- W1997455905 cites W2093679603 @default.
- W1997455905 cites W2108543631 @default.
- W1997455905 cites W2112348939 @default.
- W1997455905 cites W2115512932 @default.
- W1997455905 cites W2120217717 @default.
- W1997455905 cites W2121218667 @default.
- W1997455905 cites W2125915154 @default.
- W1997455905 cites W2127073167 @default.
- W1997455905 cites W2133491083 @default.
- W1997455905 cites W2133643647 @default.
- W1997455905 cites W2140230014 @default.
- W1997455905 cites W2140308968 @default.
- W1997455905 cites W2142416165 @default.
- W1997455905 cites W2146454701 @default.
- W1997455905 cites W2153980875 @default.
- W1997455905 cites W2159460045 @default.
- W1997455905 cites W2163529853 @default.
- W1997455905 cites W2164952355 @default.
- W1997455905 cites W2166353874 @default.
- W1997455905 cites W2169911836 @default.
- W1997455905 doi "https://doi.org/10.1534/genetics.111.131938" @default.
- W1997455905 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3241404" @default.
- W1997455905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21926300" @default.
- W1997455905 hasPublicationYear "2011" @default.
- W1997455905 type Work @default.
- W1997455905 sameAs 1997455905 @default.
- W1997455905 citedByCount "9" @default.
- W1997455905 countsByYear W19974559052013 @default.
- W1997455905 countsByYear W19974559052014 @default.
- W1997455905 countsByYear W19974559052015 @default.
- W1997455905 countsByYear W19974559052018 @default.
- W1997455905 countsByYear W19974559052019 @default.
- W1997455905 countsByYear W19974559052020 @default.
- W1997455905 countsByYear W19974559052022 @default.
- W1997455905 crossrefType "journal-article" @default.
- W1997455905 hasAuthorship W1997455905A5023165801 @default.
- W1997455905 hasAuthorship W1997455905A5031007485 @default.
- W1997455905 hasAuthorship W1997455905A5036797836 @default.
- W1997455905 hasAuthorship W1997455905A5037768394 @default.
- W1997455905 hasAuthorship W1997455905A5052529961 @default.
- W1997455905 hasAuthorship W1997455905A5058543639 @default.
- W1997455905 hasAuthorship W1997455905A5062169677 @default.
- W1997455905 hasAuthorship W1997455905A5062797658 @default.
- W1997455905 hasBestOaLocation W19974559051 @default.
- W1997455905 hasConcept C104317684 @default.
- W1997455905 hasConcept C105696609 @default.
- W1997455905 hasConcept C134935766 @default.
- W1997455905 hasConcept C143425029 @default.
- W1997455905 hasConcept C1491633281 @default.
- W1997455905 hasConcept C156719811 @default.
- W1997455905 hasConcept C23458808 @default.
- W1997455905 hasConcept C2756471 @default.
- W1997455905 hasConcept C29537977 @default.
- W1997455905 hasConcept C49105822 @default.