Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997457344> ?p ?o ?g. }
- W1997457344 endingPage "878" @default.
- W1997457344 startingPage "872" @default.
- W1997457344 abstract "Matrix-assisted laser desorption/ionization mass spectrometry is a powerful analytical tool for the structural characterization of oligonucleotides and nucleic acids. Here we report the application of stable isotope labeling for the simplified characterization of ribonucleic acids (RNAs). An (18)O label is incorporated at the 3'-phosphate of oligoribonucleotides during the enzymatic processing of intact RNAs. As implemented, a buffer solution containing a 50 : 50 mixture of H(2)O and (18)O-labeled H(2)O is used during endonuclease digestion. Upon digestion, characteristic doublets representative of the isotopic distribution of oxygen are noted for those products that contain 3'-phosphate groups. This approach is used to distinguish readily endonuclease digestion products from incomplete digestion products and non-specific cleavage products. In addition, RNase digestion products containing the characteristic isotopic doublet can be selected for further characterization by post-source decay (PSD) analysis. PSD products carrying the 3'-phosphate group will appear as a doublet, thereby simplifying fragment ion assignment." @default.
- W1997457344 created "2016-06-24" @default.
- W1997457344 creator A5020818781 @default.
- W1997457344 creator A5037885044 @default.
- W1997457344 date "2003-07-22" @default.
- W1997457344 modified "2023-10-12" @default.
- W1997457344 title "Stable isotope labeling for matrix-assisted laser desorption/ionization mass spectrometry and post-source decay analysis of ribonucleic acids" @default.
- W1997457344 cites W1966940007 @default.
- W1997457344 cites W1972927753 @default.
- W1997457344 cites W1973835606 @default.
- W1997457344 cites W1975036946 @default.
- W1997457344 cites W1977943530 @default.
- W1997457344 cites W1984482878 @default.
- W1997457344 cites W1984531315 @default.
- W1997457344 cites W1984534964 @default.
- W1997457344 cites W1985428254 @default.
- W1997457344 cites W1986724278 @default.
- W1997457344 cites W1987446193 @default.
- W1997457344 cites W1992595091 @default.
- W1997457344 cites W2002445309 @default.
- W1997457344 cites W2006419756 @default.
- W1997457344 cites W2008471565 @default.
- W1997457344 cites W2016707540 @default.
- W1997457344 cites W2033413555 @default.
- W1997457344 cites W2042326593 @default.
- W1997457344 cites W2051646379 @default.
- W1997457344 cites W2054747743 @default.
- W1997457344 cites W2057830840 @default.
- W1997457344 cites W2069165930 @default.
- W1997457344 cites W2069862631 @default.
- W1997457344 cites W2076230403 @default.
- W1997457344 cites W2078754103 @default.
- W1997457344 cites W2079073421 @default.
- W1997457344 cites W2082300861 @default.
- W1997457344 cites W2082346918 @default.
- W1997457344 cites W2083553401 @default.
- W1997457344 cites W2085948670 @default.
- W1997457344 cites W2089755851 @default.
- W1997457344 cites W2100152083 @default.
- W1997457344 cites W2105176936 @default.
- W1997457344 cites W2105431696 @default.
- W1997457344 cites W2105529830 @default.
- W1997457344 cites W2105869274 @default.
- W1997457344 cites W2109446129 @default.
- W1997457344 cites W2110850678 @default.
- W1997457344 cites W2138270253 @default.
- W1997457344 cites W2154423095 @default.
- W1997457344 cites W2155060633 @default.
- W1997457344 cites W2156565222 @default.
- W1997457344 cites W2162906563 @default.
- W1997457344 cites W2406114471 @default.
- W1997457344 cites W312568510 @default.
- W1997457344 cites W4230164190 @default.
- W1997457344 cites W4238022135 @default.
- W1997457344 doi "https://doi.org/10.1002/jms.504" @default.
- W1997457344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12938108" @default.
- W1997457344 hasPublicationYear "2003" @default.
- W1997457344 type Work @default.
- W1997457344 sameAs 1997457344 @default.
- W1997457344 citedByCount "31" @default.
- W1997457344 countsByYear W19974573442012 @default.
- W1997457344 countsByYear W19974573442013 @default.
- W1997457344 countsByYear W19974573442014 @default.
- W1997457344 countsByYear W19974573442015 @default.
- W1997457344 countsByYear W19974573442017 @default.
- W1997457344 countsByYear W19974573442018 @default.
- W1997457344 countsByYear W19974573442023 @default.
- W1997457344 crossrefType "journal-article" @default.
- W1997457344 hasAuthorship W1997457344A5020818781 @default.
- W1997457344 hasAuthorship W1997457344A5037885044 @default.
- W1997457344 hasConcept C104317684 @default.
- W1997457344 hasConcept C10879258 @default.
- W1997457344 hasConcept C129312508 @default.
- W1997457344 hasConcept C150394285 @default.
- W1997457344 hasConcept C162356407 @default.
- W1997457344 hasConcept C162711632 @default.
- W1997457344 hasConcept C167050937 @default.
- W1997457344 hasConcept C178790620 @default.
- W1997457344 hasConcept C185592680 @default.
- W1997457344 hasConcept C24107716 @default.
- W1997457344 hasConcept C2777028655 @default.
- W1997457344 hasConcept C2777132085 @default.
- W1997457344 hasConcept C36674120 @default.
- W1997457344 hasConcept C40684141 @default.
- W1997457344 hasConcept C43617362 @default.
- W1997457344 hasConcept C552990157 @default.
- W1997457344 hasConcept C55493867 @default.
- W1997457344 hasConcept C67705224 @default.
- W1997457344 hasConcept C75280812 @default.
- W1997457344 hasConceptScore W1997457344C104317684 @default.
- W1997457344 hasConceptScore W1997457344C10879258 @default.
- W1997457344 hasConceptScore W1997457344C129312508 @default.
- W1997457344 hasConceptScore W1997457344C150394285 @default.
- W1997457344 hasConceptScore W1997457344C162356407 @default.
- W1997457344 hasConceptScore W1997457344C162711632 @default.
- W1997457344 hasConceptScore W1997457344C167050937 @default.
- W1997457344 hasConceptScore W1997457344C178790620 @default.
- W1997457344 hasConceptScore W1997457344C185592680 @default.