FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997465134> ?p ?o ?g. }

• W1997465134 endingPage "11289" @default.
• W1997465134 startingPage "11284" @default.
• W1997465134 abstract "We engineered short ORFs and used them to control the expression level of recombinant proteins. These short ORFs, encoding a two-amino acid peptide, were placed upstream of an ORF encoding a protein of interest. Insertion of these upstream ORFs (uORFs) resulted in suppression of protein expression. By varying the base sequence preceding the uORF, we sought to vary the translation initiation rate of the uORF and subsequently control the degree of this suppression. Using this strategy, we generated a library of RNA sequence elements that can specify protein expression over a broad range of levels. By also using multiple uORFs in series and non-AUG start codons, we were able to generate particularly low expression levels, allowing us to achieve expression levels spanning three orders of magnitude. Modeling supported a mechanism where uORFs shunt the flow of ribosomes away from the downstream protein-coding ORF. With a lower translation initiation rate at the uORF, more ribosomes “leak” past the uORF; consequently, more ribosomes are able to reach and translate the downstream ORF. We report expression control by engineering uORFs and translation initiation to be robust, predictable, and reproducible across all cell types tested. We propose control of translation initiation as a primary method of choice for tuning expression in mammalian systems." @default.
• W1997465134 created "2016-06-24" @default.
• W1997465134 creator A5001070171 @default.
• W1997465134 creator A5023572822 @default.
• W1997465134 creator A5084632268 @default.
• W1997465134 date "2013-06-24" @default.
• W1997465134 modified "2023-10-16" @default.
• W1997465134 title "Tuning gene expression with synthetic upstream open reading frames" @default.
• W1997465134 cites W1971739920 @default.
• W1997465134 cites W1976105576 @default.
• W1997465134 cites W2006906242 @default.
• W1997465134 cites W2008410413 @default.
• W1997465134 cites W2009105413 @default.
• W1997465134 cites W2015519364 @default.
• W1997465134 cites W2020828649 @default.
• W1997465134 cites W2026487666 @default.
• W1997465134 cites W2031374133 @default.
• W1997465134 cites W2033345778 @default.
• W1997465134 cites W2047360542 @default.
• W1997465134 cites W2047381200 @default.
• W1997465134 cites W2056220960 @default.
• W1997465134 cites W2079504149 @default.
• W1997465134 cites W2089218510 @default.
• W1997465134 cites W2099390806 @default.
• W1997465134 cites W2108491944 @default.
• W1997465134 cites W2136201129 @default.
• W1997465134 cites W2152052780 @default.
• W1997465134 cites W2152974197 @default.
• W1997465134 cites W2153949330 @default.
• W1997465134 cites W2154128645 @default.
• W1997465134 cites W2160834432 @default.
• W1997465134 cites W2162262059 @default.
• W1997465134 cites W4250585317 @default.
• W1997465134 cites W2000368112 @default.
• W1997465134 doi "https://doi.org/10.1073/pnas.1305590110" @default.
• W1997465134 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3710870" @default.
• W1997465134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23798422" @default.
• W1997465134 hasPublicationYear "2013" @default.
• W1997465134 type Work @default.
• W1997465134 sameAs 1997465134 @default.
• W1997465134 citedByCount "98" @default.
• W1997465134 countsByYear W19974651342013 @default.
• W1997465134 countsByYear W19974651342014 @default.
• W1997465134 countsByYear W19974651342015 @default.
• W1997465134 countsByYear W19974651342016 @default.
• W1997465134 countsByYear W19974651342017 @default.
• W1997465134 countsByYear W19974651342018 @default.
• W1997465134 countsByYear W19974651342019 @default.
• W1997465134 countsByYear W19974651342020 @default.
• W1997465134 countsByYear W19974651342021 @default.
• W1997465134 countsByYear W19974651342022 @default.
• W1997465134 countsByYear W19974651342023 @default.
• W1997465134 crossrefType "journal-article" @default.
• W1997465134 hasAuthorship W1997465134A5001070171 @default.
• W1997465134 hasAuthorship W1997465134A5023572822 @default.
• W1997465134 hasAuthorship W1997465134A5084632268 @default.
• W1997465134 hasBestOaLocation W19974651341 @default.
• W1997465134 hasConcept C104317684 @default.
• W1997465134 hasConcept C105580179 @default.
• W1997465134 hasConcept C12464233 @default.
• W1997465134 hasConcept C149364088 @default.
• W1997465134 hasConcept C166258313 @default.
• W1997465134 hasConcept C167625842 @default.
• W1997465134 hasConcept C176990463 @default.
• W1997465134 hasConcept C182325514 @default.
• W1997465134 hasConcept C2780530800 @default.
• W1997465134 hasConcept C3675279 @default.
• W1997465134 hasConcept C4718897 @default.
• W1997465134 hasConcept C47289529 @default.
• W1997465134 hasConcept C54355233 @default.
• W1997465134 hasConcept C55334214 @default.
• W1997465134 hasConcept C67705224 @default.
• W1997465134 hasConcept C86803240 @default.
• W1997465134 hasConcept C88478588 @default.
• W1997465134 hasConcept C91779695 @default.
• W1997465134 hasConceptScore W1997465134C104317684 @default.
• W1997465134 hasConceptScore W1997465134C105580179 @default.
• W1997465134 hasConceptScore W1997465134C12464233 @default.
• W1997465134 hasConceptScore W1997465134C149364088 @default.
• W1997465134 hasConceptScore W1997465134C166258313 @default.
• W1997465134 hasConceptScore W1997465134C167625842 @default.
• W1997465134 hasConceptScore W1997465134C176990463 @default.
• W1997465134 hasConceptScore W1997465134C182325514 @default.
• W1997465134 hasConceptScore W1997465134C2780530800 @default.
• W1997465134 hasConceptScore W1997465134C3675279 @default.
• W1997465134 hasConceptScore W1997465134C4718897 @default.
• W1997465134 hasConceptScore W1997465134C47289529 @default.
• W1997465134 hasConceptScore W1997465134C54355233 @default.
• W1997465134 hasConceptScore W1997465134C55334214 @default.
• W1997465134 hasConceptScore W1997465134C67705224 @default.
• W1997465134 hasConceptScore W1997465134C86803240 @default.
• W1997465134 hasConceptScore W1997465134C88478588 @default.
• W1997465134 hasConceptScore W1997465134C91779695 @default.
• W1997465134 hasIssue "28" @default.
• W1997465134 hasLocation W19974651341 @default.
• W1997465134 hasLocation W19974651342 @default.
• W1997465134 hasLocation W19974651343 @default.
• W1997465134 hasLocation W19974651344 @default.

• next