Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997475201> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1997475201 endingPage "446" @default.
- W1997475201 startingPage "446" @default.
- W1997475201 abstract "Leaf curl and yellowing symptoms on tomato, and yellow mosaic symptoms on eggplant, are frequently observed in Kanchanaburi Province, Thailand. DNA was extracted from leaves of 13 symptomatic tomato and six symptomatic eggplant samples by the method of Gilbertson et al (1). Viral DNA was amplified by polymerase chain reaction (PCR) using the begomovirus-specific degenerate primer pair PAL1v1978/PAR1c715 (3), which amplified a 1.4-kb fragment of DNA-A. All samples, except one eggplant sample, yielded the expected product. The 1.4-kb PCR products of one tomato and one eggplant sample were cloned and sequenced. Both begomoviruses from tomato and eggplant contained a DNA-B component, amplified using two degenerate primer pairs, DNABLC1/DNABLV2 and DNABLC2/DNABLV2 (2). Based on sequences of the DNA products amplified by the degenerate primer pairs, specific primers were designed to complete the DNA-A and DNA-B sequences. Computer-assisted sequence comparisons were performed with geminivirus sequences available in the laboratory at the Asian Vegetable Research and Development Center, Taiwan and in the GenBank sequence database. Both tomato (GenBank Accession Nos. AF511529 and AF511528) and eggplant (GenBank Accession Nos. AF511530 and AF511527) virus isolates contain the conserved geminivirus sequence-TAATATTAC on the DNA-A and B. Based on the high sequence identities of 99% DNA-A and 98% DNA-B, these two virus isolates are considered to be the same species. Although the genome organization of these two isolates was the same as Tomato yellow leaf curl virus Thailand (TYLCTHV; GenBank Accession Nos. X63015 and X63016), including six open reading frames (ORFs) on the DNA-A (AV1, AV2, AC1, AC2, AC3, and AC4) and two ORFs on the DNA-B (BV1 and BC1), sequence comparisons showed highest DNA-A sequence identity (73%) with Ageratum yellow vein virus from Singapore (GenBank Accession No. X74516) and highest DNA-B identity (77%) with the TYLCTHV (X63016). To our knowledge, these tomato- and eggplant-infecting viruses from Thailand constitute a distinctly novel bipartite Begomovirus species. References: (1) R. L. Gilbertson et al. J. Gen. Virol. 72:2843, 1991. (2) S. K. Green et al. Plant Dis. 85:1286, 2001. (3) M. R. Rojas et al. Plant Dis. 77:340, 1993." @default.
- W1997475201 created "2016-06-24" @default.
- W1997475201 creator A5034658876 @default.
- W1997475201 creator A5036990582 @default.
- W1997475201 creator A5038182055 @default.
- W1997475201 creator A5069287832 @default.
- W1997475201 creator A5082635039 @default.
- W1997475201 date "2003-04-01" @default.
- W1997475201 modified "2023-09-27" @default.
- W1997475201 title "Molecular Characterization of a New Begomovirus Associated with Tomato Yellow Leaf Curl and Eggplant Yellow Mosaic Diseases in Thailand" @default.
- W1997475201 doi "https://doi.org/10.1094/pdis.2003.87.4.446a" @default.
- W1997475201 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30831844" @default.
- W1997475201 hasPublicationYear "2003" @default.
- W1997475201 type Work @default.
- W1997475201 sameAs 1997475201 @default.
- W1997475201 citedByCount "25" @default.
- W1997475201 countsByYear W19974752012012 @default.
- W1997475201 countsByYear W19974752012013 @default.
- W1997475201 countsByYear W19974752012014 @default.
- W1997475201 countsByYear W19974752012015 @default.
- W1997475201 countsByYear W19974752012016 @default.
- W1997475201 countsByYear W19974752012017 @default.
- W1997475201 countsByYear W19974752012018 @default.
- W1997475201 countsByYear W19974752012019 @default.
- W1997475201 countsByYear W19974752012020 @default.
- W1997475201 countsByYear W19974752012022 @default.
- W1997475201 countsByYear W19974752012023 @default.
- W1997475201 crossrefType "journal-article" @default.
- W1997475201 hasAuthorship W1997475201A5034658876 @default.
- W1997475201 hasAuthorship W1997475201A5036990582 @default.
- W1997475201 hasAuthorship W1997475201A5038182055 @default.
- W1997475201 hasAuthorship W1997475201A5069287832 @default.
- W1997475201 hasAuthorship W1997475201A5082635039 @default.
- W1997475201 hasBestOaLocation W19974752011 @default.
- W1997475201 hasConcept C104317684 @default.
- W1997475201 hasConcept C109110057 @default.
- W1997475201 hasConcept C141231307 @default.
- W1997475201 hasConcept C15190224 @default.
- W1997475201 hasConcept C159047783 @default.
- W1997475201 hasConcept C178790620 @default.
- W1997475201 hasConcept C185592680 @default.
- W1997475201 hasConcept C2522874641 @default.
- W1997475201 hasConcept C2777563447 @default.
- W1997475201 hasConcept C2778016631 @default.
- W1997475201 hasConcept C2778053290 @default.
- W1997475201 hasConcept C2779263152 @default.
- W1997475201 hasConcept C49105822 @default.
- W1997475201 hasConcept C54355233 @default.
- W1997475201 hasConcept C552990157 @default.
- W1997475201 hasConcept C61053724 @default.
- W1997475201 hasConcept C79029880 @default.
- W1997475201 hasConcept C84148353 @default.
- W1997475201 hasConcept C86803240 @default.
- W1997475201 hasConceptScore W1997475201C104317684 @default.
- W1997475201 hasConceptScore W1997475201C109110057 @default.
- W1997475201 hasConceptScore W1997475201C141231307 @default.
- W1997475201 hasConceptScore W1997475201C15190224 @default.
- W1997475201 hasConceptScore W1997475201C159047783 @default.
- W1997475201 hasConceptScore W1997475201C178790620 @default.
- W1997475201 hasConceptScore W1997475201C185592680 @default.
- W1997475201 hasConceptScore W1997475201C2522874641 @default.
- W1997475201 hasConceptScore W1997475201C2777563447 @default.
- W1997475201 hasConceptScore W1997475201C2778016631 @default.
- W1997475201 hasConceptScore W1997475201C2778053290 @default.
- W1997475201 hasConceptScore W1997475201C2779263152 @default.
- W1997475201 hasConceptScore W1997475201C49105822 @default.
- W1997475201 hasConceptScore W1997475201C54355233 @default.
- W1997475201 hasConceptScore W1997475201C552990157 @default.
- W1997475201 hasConceptScore W1997475201C61053724 @default.
- W1997475201 hasConceptScore W1997475201C79029880 @default.
- W1997475201 hasConceptScore W1997475201C84148353 @default.
- W1997475201 hasConceptScore W1997475201C86803240 @default.
- W1997475201 hasIssue "4" @default.
- W1997475201 hasLocation W19974752011 @default.
- W1997475201 hasLocation W19974752012 @default.
- W1997475201 hasOpenAccess W1997475201 @default.
- W1997475201 hasPrimaryLocation W19974752011 @default.
- W1997475201 hasRelatedWork W1966914617 @default.
- W1997475201 hasRelatedWork W1997475201 @default.
- W1997475201 hasRelatedWork W2043872272 @default.
- W1997475201 hasRelatedWork W2051331800 @default.
- W1997475201 hasRelatedWork W2059849134 @default.
- W1997475201 hasRelatedWork W2069441286 @default.
- W1997475201 hasRelatedWork W2117684347 @default.
- W1997475201 hasRelatedWork W2133334446 @default.
- W1997475201 hasRelatedWork W3097036579 @default.
- W1997475201 hasRelatedWork W4224293001 @default.
- W1997475201 hasVolume "87" @default.
- W1997475201 isParatext "false" @default.
- W1997475201 isRetracted "false" @default.
- W1997475201 magId "1997475201" @default.
- W1997475201 workType "article" @default.