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- W1997480223 abstract "We evaluated the influence of mode of delivery on the hematopoietic stem cell (HSCs) content (colony-forming cells, CFC, and the number of CD34+ cells) of 120 consecutive umbilical cord blood (UCB) units collected in our bank iin 1999. Ninety-eight units were collected after vaginal delivery (VD) and 22 after cesarean section (CS). Table 1 Table 1 Table 1 CFC/μl CD34+ cells/μl CFC/CD34+ cells VD-UCB 41.4 (21.2) 68.3 (38) 0.67 (0.34) CS-UCB 42.2 (22.4) 95.2 (38) 0.45 (0.19) t-test p = 0.403 p = 0.003 p = 0.005 Open table in a new tab reports mean and SD of the HSCs content of VD and CS units and the results of the statistical analysis: CS-UCB showed a significant higher number of CD34+ cell counts not associated with a corresponding increment of CFC counts. In order to expand this observation we evaluated in another series of 22 VD-UCB and 12 CS-UCB, the percentages of apoptotic (Annexin-V+/7-aminoactinomicin−) and necrotic (Annexin-V+/7-aminoactinomicin+) CD34+ cells by flow-cytometry. Table 2 Table 2 Table 2 % apoptotic CD34+ cells % necrotic CD34+ cells VD-UCB 4.7 (4.3) 6.6 (3.9) CS-UCB 2.6 (4.1) 13.2 (7.7) t-test p = 0.196 p = 0.002 Open table in a new tab reports the results (mean and SD) and statistical analysis of this part of the study. Our data suggest that increased % of necrotic CD34+ cells may be the cause of decreased clonogenic potential of CS-UCB." @default.
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- W1997480223 date "2000-07-01" @default.
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- W1997480223 title "The influence of mode of delivery on hematopoietic stem cells in umbilical cord blood collections" @default.
- W1997480223 doi "https://doi.org/10.1016/s0301-472x(00)00317-9" @default.
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