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• W1997482022 endingPage "112" @default.
• W1997482022 startingPage "103" @default.
• W1997482022 abstract "It has recently been proposed that guanine nucleotide (GTP)- binding proteins (G-proteins) are involved in transducing stimuli in olfactory receptor neurons3,43. If this is the case, G-proteins should be expressed when receptor cells first generate action potentials in response to odorants, i.e. in the rat fetus on the 16th embryonic day (E16)14. We have done an immunohistochemical study to determine when the α- and β-subunits of the stimulatory G-protein (Gs), are expressed in developing rat olfactory epithelium. The 3 primary antisera used were monospecific polyclonal antibodies generated in rabbits by immunization with synthetic peptides, the amino acid sequences of which matched a portion of the α- or β-subunits of stimulatory G-protein39. Both subunits were present in olfactory axons at E15 and in olfactory receptor cell cilia at E16, the day when cilia first sprout in these cells. As development progressed and more cilia grew, most were immunoreactive with antisera to both subunits. Examination of specimens with electron microscopic immunocytochemistry confirmed the localization. Not all cilia on a given olfactory cell were stained, in either fetal or juvenile specimens. The observation that G-proteins are expressed in cilia when action potentials are first demonstrated supports the hypothesis that G-proteins are involved in signal transduction in olfactory receptor cells." @default.
• W1997482022 created "2016-06-24" @default.
• W1997482022 creator A5066373582 @default.
• W1997482022 creator A5081771529 @default.
• W1997482022 date "1990-01-01" @default.
• W1997482022 modified "2023-09-23" @default.
• W1997482022 title "Immunohistochemical localization of guanine nucleotide-binding proteins in rat olfactory epithelium during development" @default.
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• W1997482022 doi "https://doi.org/10.1016/0165-3806(90)90262-w" @default.
• W1997482022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2105176" @default.
• W1997482022 hasPublicationYear "1990" @default.
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