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- W1997500614 abstract "The cholesterol content of rat liver plasma membranes was manipulated using either cholesterol‐free or cholesterol‐enriched liposomes. Removal of cholesterol from the membranes led to a marked increase in 5′‐nucleotidase activity. However, increase in cholesterol content failed to exert any significant effect on 5′‐nucleotidase activity. Arrhenius plots of the activity of the native enzyme exhibited a break at around 28°C with the activation energy of the reaction less above this temperature than below. In cholesterol‐depleted membranes a single break at around 26°C was observed with activation energies greater above this temperature than below it. In cholesterol‐enriched membranes Arrhenius plots were linear over the range examined. It is suggested that the lipid environment of the external half of the bilayer only influences 5′‐nucleotidase activity in these membranes and that cholesterol exerts controlling effects on both the activity and conformation of the enzyme in native membranes." @default.
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- W1997500614 date "1983-06-27" @default.
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- W1997500614 title "5′-Nucleotidase is activated upon cholesterol-depletion of liver plasma membranes" @default.
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- W1997500614 doi "https://doi.org/10.1016/0014-5793(83)81118-1" @default.
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