FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997508715> ?p ?o ?g. }

• W1997508715 endingPage "157" @default.
• W1997508715 startingPage "147" @default.
• W1997508715 abstract "The cell line MD-IGF-1, containing an ovine IGF-1 cDNA driven by the mouse mammary tumor virus-long terminal repeat (MMTV-LTR) promoter, was used to study expression of IGF-1 linked to the MMTV-LTR in bovine mammary epithelial cells in response to various hormonal and substratum stimuli. Acute sensitivity of the MMTV-LTR promoter to glucocorticoids and sex steroids was ascertained by transient transfection of parental MAC-T cells with an MMTV-CAT construct. Specifically, CAT activity was induced by glucocorticoids, but not by 17 beta-estradiol or progesterone. Induction of MD-IGF-1 cells with dexamethasone (DEX) alone triggered a 29.5-fold increase in secretion of recombinant IGF-1 (348.9 vs 11.8 pg/micrograms DNA), and stimulated a 1.7-fold increase in total DNA within 72 h. Growth of MD-IGF-1 cells was enhanced by exogenous IGF-1, insulin, and TGF-alpha. In contrast, TGF-beta inhibited cell proliferation, while epidermal growth factor, estrogen, progesterone, and testosterone had no effect. Extracellular matrix from the Engelbreth-Holm-Swarm (EHS) tumor, in the presence of DEX, prolactin (PRL), and insulin stimulated a 29.4-fold increase in secretion of IGF-1 (591.9 pg/microgram DNA), compared with cells in absence of hormones (20.1 pg/micrograms DNA). EHS and DEX plus PRL triggered a 63.2-fold increase in IGF-1 secretion (689.1 pg/micrograms DNA), compared with MD-IGF-1 cells cultured on plastic (10.9 pg/micrograms DNA), in the absence of hormones. These data indicate that the MMTV-LTR is regulated by both lactogenic hormones and extracellular matrix in MD-IGF-1 cells and that the MMTV-LTR may be a useful regulatory element for targeting expression of foreign proteins in bovine mammary epithelial cells." @default.
• W1997508715 created "2016-06-24" @default.
• W1997508715 creator A5013811104 @default.
• W1997508715 creator A5016597766 @default.
• W1997508715 creator A5019125489 @default.
• W1997508715 creator A5026704782 @default.
• W1997508715 creator A5028288352 @default.
• W1997508715 creator A5028968226 @default.
• W1997508715 creator A5086485401 @default.
• W1997508715 date "1993-10-01" @default.
• W1997508715 modified "2023-09-24" @default.
• W1997508715 title "Lactogenic hormones and extracellular matrix regulate expression of IGF-1 linked to MMTV-LTR in mammary epithelial cells" @default.
• W1997508715 cites W1519416684 @default.
• W1997508715 cites W1748552538 @default.
• W1997508715 cites W1794613013 @default.
• W1997508715 cites W1896222284 @default.
• W1997508715 cites W1906450972 @default.
• W1997508715 cites W1964547978 @default.
• W1997508715 cites W1966304710 @default.
• W1997508715 cites W1972976329 @default.
• W1997508715 cites W1973227330 @default.
• W1997508715 cites W1977779461 @default.
• W1997508715 cites W1979419333 @default.
• W1997508715 cites W1986846519 @default.
• W1997508715 cites W1990169853 @default.
• W1997508715 cites W1992668278 @default.
• W1997508715 cites W1998280478 @default.
• W1997508715 cites W2000346045 @default.
• W1997508715 cites W2011761081 @default.
• W1997508715 cites W2011808338 @default.
• W1997508715 cites W2012796972 @default.
• W1997508715 cites W2020834476 @default.
• W1997508715 cites W2028861404 @default.
• W1997508715 cites W2048287289 @default.
• W1997508715 cites W2052141606 @default.
• W1997508715 cites W2052148868 @default.
• W1997508715 cites W205313094 @default.
• W1997508715 cites W2056557947 @default.
• W1997508715 cites W2056593076 @default.
• W1997508715 cites W2073865988 @default.
• W1997508715 cites W2074429640 @default.
• W1997508715 cites W2075449661 @default.
• W1997508715 cites W2079331878 @default.
• W1997508715 cites W2081854624 @default.
• W1997508715 cites W2082405573 @default.
• W1997508715 cites W2091483298 @default.
• W1997508715 cites W2113362967 @default.
• W1997508715 cites W2140481202 @default.
• W1997508715 cites W2182573189 @default.
• W1997508715 cites W2280018781 @default.
• W1997508715 cites W2465501540 @default.
• W1997508715 cites W4239773677 @default.
• W1997508715 doi "https://doi.org/10.1016/0303-7207(93)90105-s" @default.
• W1997508715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8276130" @default.
• W1997508715 hasPublicationYear "1993" @default.
• W1997508715 type Work @default.
• W1997508715 sameAs 1997508715 @default.
• W1997508715 citedByCount "15" @default.
• W1997508715 countsByYear W19975087152012 @default.
• W1997508715 countsByYear W19975087152013 @default.
• W1997508715 crossrefType "journal-article" @default.
• W1997508715 hasAuthorship W1997508715A5013811104 @default.
• W1997508715 hasAuthorship W1997508715A5016597766 @default.
• W1997508715 hasAuthorship W1997508715A5019125489 @default.
• W1997508715 hasAuthorship W1997508715A5026704782 @default.
• W1997508715 hasAuthorship W1997508715A5028288352 @default.
• W1997508715 hasAuthorship W1997508715A5028968226 @default.
• W1997508715 hasAuthorship W1997508715A5086485401 @default.
• W1997508715 hasConcept C104317684 @default.
• W1997508715 hasConcept C121608353 @default.
• W1997508715 hasConcept C126322002 @default.
• W1997508715 hasConcept C134018914 @default.
• W1997508715 hasConcept C150194340 @default.
• W1997508715 hasConcept C153911025 @default.
• W1997508715 hasConcept C199491388 @default.
• W1997508715 hasConcept C2776187077 @default.
• W1997508715 hasConcept C2777164284 @default.
• W1997508715 hasConcept C2779064019 @default.
• W1997508715 hasConcept C2779777497 @default.
• W1997508715 hasConcept C28406088 @default.
• W1997508715 hasConcept C49039625 @default.
• W1997508715 hasConcept C530470458 @default.
• W1997508715 hasConcept C54009773 @default.
• W1997508715 hasConcept C54355233 @default.
• W1997508715 hasConcept C552990157 @default.
• W1997508715 hasConcept C55493867 @default.
• W1997508715 hasConcept C71315377 @default.
• W1997508715 hasConcept C71924100 @default.
• W1997508715 hasConcept C81885089 @default.
• W1997508715 hasConcept C86803240 @default.
• W1997508715 hasConcept C95444343 @default.
• W1997508715 hasConceptScore W1997508715C104317684 @default.
• W1997508715 hasConceptScore W1997508715C121608353 @default.
• W1997508715 hasConceptScore W1997508715C126322002 @default.
• W1997508715 hasConceptScore W1997508715C134018914 @default.
• W1997508715 hasConceptScore W1997508715C150194340 @default.
• W1997508715 hasConceptScore W1997508715C153911025 @default.
• W1997508715 hasConceptScore W1997508715C199491388 @default.

• next