FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997514106> ?p ?o ?g. }

• W1997514106 endingPage "251" @default.
• W1997514106 startingPage "241" @default.
• W1997514106 abstract "Treatment of excitotoxically injured organotypic hippocampal slice cultures (OHSC) with clodronate is known to result in the inhibition of microglial activation. We hypothesized that this is due to direct effects of clodronate on microglial cells, and investigated microglial proliferation in OHSC, and cytokine and NO secretion in isolated microglial cells. N-methyl-d-aspartate (NMDA) lesioning of OHSC resulted in a massive increase in the number of proliferating, bromo-desoxy-uridine (BrdU)-labeled cells that was reduced to control levels after treatment with clodronate (0.1, 1, 10 μg/ml). Triple-labeling revealed that clodronate abrogated the proliferation of both glial fibrillary acidic protein (GFAP)-labeled astrocytes and Griffonia simplicifolia isolectin B4 (IB4)-labeled microglial cells. Furthermore, isolated microglial cells were treated with clodronate after stimulation with lipopolysaccharide (LPS) or macrophage colony stimulating factor (M-CSF). Clodronate (0.01, 0.1, 1 μg/ml) significantly down-regulated the LPS-stimulated microglial secretion of tumor necrosis factor (TNF)-α, Interleukin (IL)-1β and NO, but not of IL-6. In contrast, clodronate significantly reduced the microglial IL-6-release induced by M-CSF, indicating different intracellular pathways. The number and morphology of isolated microglial cells did not change significantly after treatment with clodronate. In summary, the number of proliferating microglial cells and astrocytes after excitotoxic injury is reduced to control levels after treatment with clodronate. Furthermore, clodronate inhibits microglial secretion of proinflammatory cytokines and NO. Clodronate could therefore prove to be a useful tool in the investigation of interactions between damaged neurons and microglial cells." @default.
• W1997514106 created "2016-06-24" @default.
• W1997514106 creator A5006590266 @default.
• W1997514106 creator A5046325640 @default.
• W1997514106 creator A5052497026 @default.
• W1997514106 creator A5056357776 @default.
• W1997514106 creator A5057133727 @default.
• W1997514106 date "2004-10-01" @default.
• W1997514106 modified "2023-10-16" @default.
• W1997514106 title "Clodronate inhibits the secretion of proinflammatory cytokines and NO by isolated microglial cells and reduces the number of proliferating glial cells in excitotoxically injured organotypic hippocampal slice cultures" @default.
• W1997514106 cites W1490228662 @default.
• W1997514106 cites W1541718087 @default.
• W1997514106 cites W1591022542 @default.
• W1997514106 cites W1600123087 @default.
• W1997514106 cites W1668344921 @default.
• W1997514106 cites W1968666033 @default.
• W1997514106 cites W1976468474 @default.
• W1997514106 cites W1981834071 @default.
• W1997514106 cites W1984131196 @default.
• W1997514106 cites W1993262247 @default.
• W1997514106 cites W1994011248 @default.
• W1997514106 cites W1996898338 @default.
• W1997514106 cites W1998907409 @default.
• W1997514106 cites W2010486222 @default.
• W1997514106 cites W2020587065 @default.
• W1997514106 cites W2021563247 @default.
• W1997514106 cites W2023207814 @default.
• W1997514106 cites W2023420622 @default.
• W1997514106 cites W2028023383 @default.
• W1997514106 cites W2038781543 @default.
• W1997514106 cites W2041788967 @default.
• W1997514106 cites W2042951548 @default.
• W1997514106 cites W2048183719 @default.
• W1997514106 cites W2049625461 @default.
• W1997514106 cites W2053513357 @default.
• W1997514106 cites W2063084132 @default.
• W1997514106 cites W2064824601 @default.
• W1997514106 cites W2077468629 @default.
• W1997514106 cites W2080411212 @default.
• W1997514106 cites W2081901209 @default.
• W1997514106 cites W2082201486 @default.
• W1997514106 cites W2084518691 @default.
• W1997514106 cites W2085220227 @default.
• W1997514106 cites W2085716611 @default.
• W1997514106 cites W2085767802 @default.
• W1997514106 cites W2089389165 @default.
• W1997514106 cites W2090603105 @default.
• W1997514106 cites W2095376056 @default.
• W1997514106 cites W2096182847 @default.
• W1997514106 cites W2104660263 @default.
• W1997514106 cites W2105544528 @default.
• W1997514106 cites W2109529667 @default.
• W1997514106 cites W2109759840 @default.
• W1997514106 cites W2144957471 @default.
• W1997514106 cites W2146670425 @default.
• W1997514106 cites W2159746265 @default.
• W1997514106 cites W2336020536 @default.
• W1997514106 cites W54816364 @default.
• W1997514106 doi "https://doi.org/10.1016/j.expneurol.2004.06.010" @default.
• W1997514106 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15380476" @default.
• W1997514106 hasPublicationYear "2004" @default.
• W1997514106 type Work @default.
• W1997514106 sameAs 1997514106 @default.
• W1997514106 citedByCount "45" @default.
• W1997514106 countsByYear W19975141062012 @default.
• W1997514106 countsByYear W19975141062013 @default.
• W1997514106 countsByYear W19975141062014 @default.
• W1997514106 countsByYear W19975141062015 @default.
• W1997514106 countsByYear W19975141062017 @default.
• W1997514106 countsByYear W19975141062018 @default.
• W1997514106 countsByYear W19975141062019 @default.
• W1997514106 countsByYear W19975141062020 @default.
• W1997514106 countsByYear W19975141062021 @default.
• W1997514106 countsByYear W19975141062022 @default.
• W1997514106 countsByYear W19975141062023 @default.
• W1997514106 crossrefType "journal-article" @default.
• W1997514106 hasAuthorship W1997514106A5006590266 @default.
• W1997514106 hasAuthorship W1997514106A5046325640 @default.
• W1997514106 hasAuthorship W1997514106A5052497026 @default.
• W1997514106 hasAuthorship W1997514106A5056357776 @default.
• W1997514106 hasAuthorship W1997514106A5057133727 @default.
• W1997514106 hasConcept C134018914 @default.
• W1997514106 hasConcept C164027704 @default.
• W1997514106 hasConcept C17991360 @default.
• W1997514106 hasConcept C185592680 @default.
• W1997514106 hasConcept C203014093 @default.
• W1997514106 hasConcept C204232928 @default.
• W1997514106 hasConcept C2776914184 @default.
• W1997514106 hasConcept C2777542381 @default.
• W1997514106 hasConcept C2778082330 @default.
• W1997514106 hasConcept C2778690821 @default.
• W1997514106 hasConcept C2779830541 @default.
• W1997514106 hasConcept C2780495277 @default.
• W1997514106 hasConcept C2780758401 @default.
• W1997514106 hasConcept C2780884517 @default.
• W1997514106 hasConcept C529278444 @default.
• W1997514106 hasConcept C55493867 @default.
• W1997514106 hasConcept C86803240 @default.

• next