FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997518685> ?p ?o ?g. }

• W1997518685 endingPage "247" @default.
• W1997518685 startingPage "233" @default.
• W1997518685 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 52:233-247 (2002) - doi:10.3354/dao052233 Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria N. A. Stokes*, L. M. Ragone Calvo, K. S. Reece, E. M. Burreson School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, Virginia 23062, USA *Email: stokes@vims.edu ABSTRACT: Quahog Parasite Unknown (QPX) is a protistan parasite that causes disease and mortality in the hard clam Mercenaria mercenaria. PCR primers and DNA oligonucleotide probes were designed and evaluated for sensitivity and specificity for the QPX organism specifically and for the phylum Labyrinthulomycota in general. The best performing QPX-specific primer pair amplified a 665 bp region of the QPX small-subunit ribosomal DNA (SSU rDNA) and detected as little as 1 fg cloned QPX SSU rDNA and 20 fg QPX genomic DNA. The primers did not amplify DNA of uninfected hard clams M. mercenaria or of the thraustochytrids Schizochytrium aggregatum, Thraustochytrium aureum, and T. striatum. The general labyrinthulomycete primers, which were designed to offer broader specificity than the QPX primers, amplified a 435 bp region of SSU rDNA from QPX, and a 436 to 437 bp region of SSU rDNA from S. aggregatum, T. aureum, and T. striatum, but did not amplify that of the clam M. mercenaria. Field validation of the QPX-specific primer pair, through comparative sampling of 224 clams collected over a 16 mo period from a QPX endemic site in Virginia, USA, indicated that the PCR assay is equivalent to histological diagnosis if initially negative PCR products are reamplified. Oligonucleotide DNA probes specific for QPX and the phylum Labyrinthulomycota were evaluated for in situ hybridization assays of cell smears or paraffin-embedded tissues. Two DNA probes for QPX offered limited sensitivity when used independently; however, when used together as a probe cocktail, sensitivity was greatly enhanced. The probe cocktail hybridized to putative QPX organisms in tissues of hard clams collected from Virginia, New Jersey, Massachusetts and Canada, suggesting that the QPX organisms in these areas are either very closely related or the same species. The QPX probe cocktail did not hybridize with clam tissue or with the thraustochytrids S. aggregatum, T. aureum, and T. striatum. The labyrinthulomycete DNA probe hybridized with QPX and the 3 thraustochytrids, with no background hybridization to clam tissue. SSU rDNA sequences were obtained for the putative QPX organisms from geographically distinct sites. Phylogenetic analyses based on the QPX and Labyrinthulomycota sequences confirmed earlier reports that QPX is a member of this phylum, but could not definitively demonstrate that all of the QPX organisms were the same species. KEY WORDS: Hard clams · Disease · QPX · Molecular techniques · PCR · Probe Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 52, No. 3. Online publication date: December 10, 2002 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2002 Inter-Research." @default.
• W1997518685 created "2016-06-24" @default.
• W1997518685 creator A5001094147 @default.
• W1997518685 creator A5013833044 @default.
• W1997518685 creator A5060729060 @default.
• W1997518685 creator A5089738550 @default.
• W1997518685 date "2002-01-01" @default.
• W1997518685 modified "2023-10-01" @default.
• W1997518685 title "Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria" @default.
• W1997518685 cites W1576743454 @default.
• W1997518685 cites W1964024454 @default.
• W1997518685 cites W1964124961 @default.
• W1997518685 cites W1965278285 @default.
• W1997518685 cites W1965720743 @default.
• W1997518685 cites W1973933412 @default.
• W1997518685 cites W1983170353 @default.
• W1997518685 cites W1985222265 @default.
• W1997518685 cites W1987232494 @default.
• W1997518685 cites W1990650765 @default.
• W1997518685 cites W2000481254 @default.
• W1997518685 cites W2018870620 @default.
• W1997518685 cites W2026114853 @default.
• W1997518685 cites W2037800182 @default.
• W1997518685 cites W2040124943 @default.
• W1997518685 cites W2044793886 @default.
• W1997518685 cites W2050222987 @default.
• W1997518685 cites W2055924220 @default.
• W1997518685 cites W2056952233 @default.
• W1997518685 cites W2066771237 @default.
• W1997518685 cites W2071980676 @default.
• W1997518685 cites W2079257090 @default.
• W1997518685 cites W2095149199 @default.
• W1997518685 cites W2106882534 @default.
• W1997518685 cites W2123657410 @default.
• W1997518685 cites W2134416046 @default.
• W1997518685 cites W2154044609 @default.
• W1997518685 cites W2156422777 @default.
• W1997518685 cites W2158714788 @default.
• W1997518685 cites W2180537156 @default.
• W1997518685 cites W2314728303 @default.
• W1997518685 cites W2318530218 @default.
• W1997518685 cites W2324776623 @default.
• W1997518685 cites W2404292611 @default.
• W1997518685 cites W2885627839 @default.
• W1997518685 cites W2965090072 @default.
• W1997518685 cites W3217097258 @default.
• W1997518685 cites W2748384280 @default.
• W1997518685 doi "https://doi.org/10.3354/dao052233" @default.
• W1997518685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12553451" @default.
• W1997518685 hasPublicationYear "2002" @default.
• W1997518685 type Work @default.
• W1997518685 sameAs 1997518685 @default.
• W1997518685 citedByCount "56" @default.
• W1997518685 countsByYear W19975186852012 @default.
• W1997518685 countsByYear W19975186852013 @default.
• W1997518685 countsByYear W19975186852014 @default.
• W1997518685 countsByYear W19975186852015 @default.
• W1997518685 countsByYear W19975186852016 @default.
• W1997518685 countsByYear W19975186852017 @default.
• W1997518685 countsByYear W19975186852018 @default.
• W1997518685 countsByYear W19975186852019 @default.
• W1997518685 countsByYear W19975186852020 @default.
• W1997518685 countsByYear W19975186852021 @default.
• W1997518685 countsByYear W19975186852022 @default.
• W1997518685 countsByYear W19975186852023 @default.
• W1997518685 crossrefType "journal-article" @default.
• W1997518685 hasAuthorship W1997518685A5001094147 @default.
• W1997518685 hasAuthorship W1997518685A5013833044 @default.
• W1997518685 hasAuthorship W1997518685A5060729060 @default.
• W1997518685 hasAuthorship W1997518685A5089738550 @default.
• W1997518685 hasBestOaLocation W19975186851 @default.
• W1997518685 hasConcept C104317684 @default.
• W1997518685 hasConcept C178790620 @default.
• W1997518685 hasConcept C185592680 @default.
• W1997518685 hasConcept C18903297 @default.
• W1997518685 hasConcept C193252679 @default.
• W1997518685 hasConcept C207812311 @default.
• W1997518685 hasConcept C2776327930 @default.
• W1997518685 hasConcept C2777563447 @default.
• W1997518685 hasConcept C54355233 @default.
• W1997518685 hasConcept C86803240 @default.
• W1997518685 hasConcept C90856448 @default.
• W1997518685 hasConceptScore W1997518685C104317684 @default.
• W1997518685 hasConceptScore W1997518685C178790620 @default.
• W1997518685 hasConceptScore W1997518685C185592680 @default.
• W1997518685 hasConceptScore W1997518685C18903297 @default.
• W1997518685 hasConceptScore W1997518685C193252679 @default.
• W1997518685 hasConceptScore W1997518685C207812311 @default.
• W1997518685 hasConceptScore W1997518685C2776327930 @default.
• W1997518685 hasConceptScore W1997518685C2777563447 @default.
• W1997518685 hasConceptScore W1997518685C54355233 @default.
• W1997518685 hasConceptScore W1997518685C86803240 @default.
• W1997518685 hasConceptScore W1997518685C90856448 @default.
• W1997518685 hasLocation W19975186851 @default.
• W1997518685 hasLocation W19975186852 @default.
• W1997518685 hasLocation W19975186853 @default.
• W1997518685 hasOpenAccess W1997518685 @default.
• W1997518685 hasPrimaryLocation W19975186851 @default.

• next