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• W1997560717 abstract "The effect of oscillating dissolved oxygen tension (DOT) on the metabolism of Kluyveromyces marxianus was investigated, particularly on the productivity of the enzyme β-galactosidase or lactase (EC 3.2.1.23). Sinusoidal DOT oscillations were established by manipulating the inflowing gases (nitrogen and oxygen), at wave periods of 300, 600 and 1200 s and DOT ranging from 0 to 20%. Cultures carried out at different constant DOT ranging from 0 to 60% of air saturation were used for comparison. Oscillations at the shortest period tested (i.e., 300 s) resulted in higher specific and volumetric enzyme activity (2800 U/g and 31,700 U/l, respectively) and ethanol concentration (12.3 g/l) compared with cultures carried out at longer wave periods (600 and 1200 s) and those cultures carried out at constant DOT (0, 1, 5 and 20%). Final biomass concentration was not affected by oscillating DOT (12.2±0.7 g/l) and specific growth rate was slightly affected. These findings suggest that some non-ideal DOT control conditions can be advantageous for the case of lactase production, leading to increases in the enzyme volumetric or specific activity, without affecting maximum biomass concentration." @default.
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• W1997560717 date "2005-02-01" @default.
• W1997560717 modified "2023-10-09" @default.
• W1997560717 title "Production of β-galactosidase by Kluyveromyces marxianus under oscillating dissolved oxygen tension" @default.
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• W1997560717 doi "https://doi.org/10.1016/j.procbio.2004.02.001" @default.
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