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- W1997570645 abstract "1. Relative incorporation of 3H-leucine in vivo into pig liver proteins expressed as counts/min/ per mg protein, was 4.0 times greater than that incorporated into skeletal muscle proteins after the administration of the labelled amino acid. RNA concentration was 4.4 times greater in the liver than in the muscle. 2. Relative incorporation of C14-leucine into proteins by rat liver post-mitochondrial fraction in cell-free amino acid incorporating system was 2.8 and 4.1 times greater than that incorporated into liver and muscle proteins by pig liver and muscle post-mitochondrial fractions. 3. Amino acid incorporating activity of pig liver post-mitochondrial fraction could be destroyed by incubating the preparations with 0.2 fig/red pancreatic ribonuclease. Homogenization of pig liver in rat liver cell-sap (which exhibits relatively high ribonuclease inhibitor activity) in the presence and absence of yeast RNA increased the relative incorporation by 30% and 20%, respectively. 4. The relationship between RNA concentration and intensity of protein synthesis and the difficulties encountered in comparing amino acid incorporating activity of the liver and muscle post-mitochondrial preparations in cell-free systems have been discussed." @default.
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- W1997570645 date "1977-01-01" @default.
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- W1997570645 title "Relative in vivo and in vitro incorporation of labelled leucine into pig liver and muscle proteins" @default.
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- W1997570645 doi "https://doi.org/10.1016/0020-711x(77)90136-7" @default.
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