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- W1997579069 abstract "We developed a novel whole blood assay to assess the effects of immunosuppressants on lymphocyte apoptosis.Peripheral blood for six experiments for each drug was treated either with cyclosporin A (CsA), mycophenolate acid (MPA), tacrolimus (TRL) or rapamycin (RAPA). Whole blood was stimulated with different concentrations of staurosporine (0-5 microM) for 24 h. Using FACS, apoptosis were measured by Annexin V expression (%cells +/- SEM).MPA, RAPA, TRL, but not CsA, increased (P < 0.05) apoptotic cells (MPA:20.9 +/- 3.7; RAPA:17.5 +/- 3.3; TRL:16.4 +/- 2.9 vs. control:15.2 +/- 2.8).This new whole blood assay exhibited that MPA has a stronger effect on apoptosis than RAPA and TRL, whereas CsA had no effect." @default.
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- W1997579069 date "2006-01-01" @default.
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- W1997579069 title "Novel apoptosis assay to analyze immunosuppression" @default.
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- W1997579069 doi "https://doi.org/10.1002/cyto.a.20218" @default.
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