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- W1997582914 abstract "A stable and permanent integration of miniature packed bed separation columns into microfluidic systems is a major issue in nano liquid chromatography. Various approaches like differently shaped retaining elements or the use of key stone effect have been investigated. We show a flexible integration of miniature packed bed separation columns into microfluidic chips utilising common HPLC material achieved by laser-assisted generation of narrow, photopolymerised frits. The generated retaining elements serve as an in- and outlet frits for the columns. An optimised pre-polymeric solution, consisting of butyl acrylates and a porogen, allows a precise fabrication of frit-type structures with lengths of less than 100 μm and the capability to withstand common slurry packing pressures of more than 250 bar. The separation of seven polycyclic aromatic hydrocarbons by pressure-driven, reversed-phase chromatography proves the high quality of the created chromatographic column inside a glass chip. Plate heights down to 2.9 were achieved and extremely fast separations with sub-second peak widths were performed in isocratic and gradient elution modes on very short columns (≤25 mm)." @default.
- W1997582914 created "2016-06-24" @default.
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- W1997582914 date "2014-11-01" @default.
- W1997582914 modified "2023-09-27" @default.
- W1997582914 title "High-performance liquid chromatography on glass chips using precisely defined porous polymer monoliths as particle retaining elements" @default.
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- W1997582914 doi "https://doi.org/10.1016/j.chroma.2014.10.008" @default.
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