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- W1997589128 abstract "Single, standard doses of the carcinogenic azo dye 3-methyl-4′-dimethyl-aminoazobenzene were administered to albino rats by injection. At different periods after the injections, the livers were homogenized and fractionated by differential centrifugation. The cytoplasmic fractions obtained were separated into subfractions by a technique used previously in analogous experiments with labelled amino acids. Finally, the contents of protein-bound azo dye were determined in the subfractions obtained. While the incorporation of the azo dye into proteins was distributed between the whole particulate and non-particulate cytoplasmic fractions in approximately the same way as was the incorporation of labeled amino acids, the two incorporation processes were divergent in the subfractions of the particles. While the amino acid incorporation was positively correlated to the RNA-contents of the subfractions, a negative correlation was observed in the case of azo dye incorporation. The results suggest that the azo dye incorporation is mediated by an enzymatic mechanism independent of the amino acid incorporation mechanism. The experiments are consistent with the notion that the detoxicating enzymatic system in liver microsomes may be involved in the formation of the azo dye-protein complexes." @default.
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- W1997589128 date "1956-06-01" @default.
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- W1997589128 title "The distribution of protein-bound azo dye in subfractions of liver cytoplasm fractions" @default.
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- W1997589128 doi "https://doi.org/10.1016/0014-4827(56)90047-7" @default.
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