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- W1997596095 abstract "Direct in situ single copy PCR (DISC-PCR) was modified to increase signal detection efficiency and physically assign two porcine microsatellites (MS), Sw552 and Sw137, to chromosome 1p at Flpter 0.1-0.2 and Flpter 0.01-0.2, respectively. These are the first two MS localized to chromosome 1p by an in situ method. The modifications of the DISC-PCR procedure included use of buffered formalin and pepsin as pre-reaction treatments, and tetramethylbenzidine (TMB) for signal detection. The detection efficiency of the modified method is twofold higher than that of the original technique." @default.
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- W1997596095 date "1998-01-01" @default.
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- W1997596095 title "Physical assignment of two porcine microsatellites to the short arm of chromosome 1 by modified DISC-PCR." @default.
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