Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997597284> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1997597284 endingPage "562" @default.
- W1997597284 startingPage "559" @default.
- W1997597284 abstract "Abstract Non‐radioactive detection of mRNA with in situ hybridization histochemistry has emerged as an important new technology for the study of gene expression. Quantitative in situ hybridization studies have generally relied upon counting of autoradiographic grains in the emulsion overlying cells containing hybridized, radioactively labeled probe. However, such high resolution studies require tedious grain counting over individual cells, frequently in addition to weeks of exposure to nuclear emulsion. The present report describes a quantitative, non‐radioactive approach to the detection of a specific mRNA in the brain with the advantages of comparatively rapid tissue processing and computerized image analysis. The validity of this approach was tested by measuring the haloperidol‐induced increase in the level of preproenkephalin mRNA in striatal sections of the rat brain using an RNA probe labeled with digoxigenin‐11‐UTP. Detection of probe hybridized to tissue sections was carried out enzymatically following complex formation with an antidigoxigenin‐alkaline phosphatase conjugate. Using computerized image analysis, it was found that chronic treatment of rats with haloperidol resulted in a 50 ± 6% increase in striatal neuronal optical density, a value in good agreement with previous studies using low‐resolution radioactive methods, showing a 30–80% increase in striatal preproenkephalin mRNA hybridization signal." @default.
- W1997597284 created "2016-06-24" @default.
- W1997597284 creator A5003942601 @default.
- W1997597284 creator A5004338485 @default.
- W1997597284 creator A5023875259 @default.
- W1997597284 creator A5028921090 @default.
- W1997597284 creator A5030081188 @default.
- W1997597284 creator A5064259824 @default.
- W1997597284 date "1991-12-01" @default.
- W1997597284 modified "2023-10-18" @default.
- W1997597284 title "Quantitative non-radioactive in situ hybridization of preproenkephalin mRNA with digoxigenin-labeled cRNA probes" @default.
- W1997597284 cites W1529166175 @default.
- W1997597284 cites W1986075380 @default.
- W1997597284 cites W1991206010 @default.
- W1997597284 cites W2015063879 @default.
- W1997597284 cites W2025314368 @default.
- W1997597284 cites W2040714787 @default.
- W1997597284 cites W2056820280 @default.
- W1997597284 cites W2062467837 @default.
- W1997597284 cites W2095956820 @default.
- W1997597284 cites W2115280754 @default.
- W1997597284 doi "https://doi.org/10.1002/ar.1092310417" @default.
- W1997597284 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1793181" @default.
- W1997597284 hasPublicationYear "1991" @default.
- W1997597284 type Work @default.
- W1997597284 sameAs 1997597284 @default.
- W1997597284 citedByCount "24" @default.
- W1997597284 countsByYear W19975972842012 @default.
- W1997597284 countsByYear W19975972842013 @default.
- W1997597284 countsByYear W19975972842014 @default.
- W1997597284 countsByYear W19975972842017 @default.
- W1997597284 countsByYear W19975972842019 @default.
- W1997597284 countsByYear W19975972842020 @default.
- W1997597284 countsByYear W19975972842023 @default.
- W1997597284 crossrefType "journal-article" @default.
- W1997597284 hasAuthorship W1997597284A5003942601 @default.
- W1997597284 hasAuthorship W1997597284A5004338485 @default.
- W1997597284 hasAuthorship W1997597284A5023875259 @default.
- W1997597284 hasAuthorship W1997597284A5028921090 @default.
- W1997597284 hasAuthorship W1997597284A5030081188 @default.
- W1997597284 hasAuthorship W1997597284A5064259824 @default.
- W1997597284 hasConcept C104317684 @default.
- W1997597284 hasConcept C105580179 @default.
- W1997597284 hasConcept C129312508 @default.
- W1997597284 hasConcept C145169222 @default.
- W1997597284 hasConcept C153911025 @default.
- W1997597284 hasConcept C160160445 @default.
- W1997597284 hasConcept C178790620 @default.
- W1997597284 hasConcept C181199279 @default.
- W1997597284 hasConcept C185592680 @default.
- W1997597284 hasConcept C2776552150 @default.
- W1997597284 hasConcept C2777822432 @default.
- W1997597284 hasConcept C2778837531 @default.
- W1997597284 hasConcept C55493867 @default.
- W1997597284 hasConcept C81439078 @default.
- W1997597284 hasConcept C86803240 @default.
- W1997597284 hasConceptScore W1997597284C104317684 @default.
- W1997597284 hasConceptScore W1997597284C105580179 @default.
- W1997597284 hasConceptScore W1997597284C129312508 @default.
- W1997597284 hasConceptScore W1997597284C145169222 @default.
- W1997597284 hasConceptScore W1997597284C153911025 @default.
- W1997597284 hasConceptScore W1997597284C160160445 @default.
- W1997597284 hasConceptScore W1997597284C178790620 @default.
- W1997597284 hasConceptScore W1997597284C181199279 @default.
- W1997597284 hasConceptScore W1997597284C185592680 @default.
- W1997597284 hasConceptScore W1997597284C2776552150 @default.
- W1997597284 hasConceptScore W1997597284C2777822432 @default.
- W1997597284 hasConceptScore W1997597284C2778837531 @default.
- W1997597284 hasConceptScore W1997597284C55493867 @default.
- W1997597284 hasConceptScore W1997597284C81439078 @default.
- W1997597284 hasConceptScore W1997597284C86803240 @default.
- W1997597284 hasIssue "4" @default.
- W1997597284 hasLocation W19975972841 @default.
- W1997597284 hasLocation W19975972842 @default.
- W1997597284 hasOpenAccess W1997597284 @default.
- W1997597284 hasPrimaryLocation W19975972841 @default.
- W1997597284 hasRelatedWork W1590549673 @default.
- W1997597284 hasRelatedWork W2007568841 @default.
- W1997597284 hasRelatedWork W2026653168 @default.
- W1997597284 hasRelatedWork W2031698083 @default.
- W1997597284 hasRelatedWork W2088139568 @default.
- W1997597284 hasRelatedWork W2105203397 @default.
- W1997597284 hasRelatedWork W2137804218 @default.
- W1997597284 hasRelatedWork W2323003408 @default.
- W1997597284 hasRelatedWork W62845753 @default.
- W1997597284 hasRelatedWork W657236858 @default.
- W1997597284 hasVolume "231" @default.
- W1997597284 isParatext "false" @default.
- W1997597284 isRetracted "false" @default.
- W1997597284 magId "1997597284" @default.
- W1997597284 workType "article" @default.