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• W1997598952 abstract "The mRNA coding for the small apo-Very Low Density Lipoprotein (apo-VLDLII) from chicken serum was highly enriched by oligo(dT) chromatography and preparative gel electrophoresis of estrogenised liver RNA. Double-stranded cDNA was synthesised by the subsequent actions of reverse transcriptase and DNA polymerase, and used for a preliminary characterisation of the structural gene. Molecular cloning of dC-tailed ds-cDNA into the Pst I site of plasmid pBR 322 yielded several recombinant clones. Five chimeric DNAs were selected and characterised by restriction enzyme mapping and electron microscopy of R-loops. At least two of them (pVLDL 3.33 and pVLDLII 4.82) contain an almost full-length ds-transcript of VLDLII mRNA in which no more than 10–20 bases at the 5′- end are missing." @default.
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• W1997598952 date "1979-01-01" @default.
• W1997598952 modified "2023-09-23" @default.
• W1997598952 title "Purification of the mRNA for chicken very low density lipoproteirtIIand molecular cloning of its full-length double-stranded cDNA" @default.
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• W1997598952 doi "https://doi.org/10.1093/nar/7.8.2147" @default.
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