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- W1997604450 abstract "Some physico-chemical properties of double-stranded DNA of bacteriophage ΦX174 have been studied. Double-stranded ΦX-DNA, formed in phage ΦX174-infected Escherichia coli C in the presence of chloramphenicol, was purified on a column of esterified albumin-celite. Upon ultracentrifugation in 1 M NaCl of a purified preparation of this double-stranded ΦX-DNA two components were observed with s20,w values of 21.4 S (Component I) and 17.4 S (Component II), respectively. After alkaline denaturation component I was converted into a structure with an s20,w value of 53 S in 1 M NaCl (pH 12.8) while Component II was converted into a mixture of circular single-stranded DNA (18.4 S) and linear single-stranded DNA (14.3 S). Both Components I and II are infectious for spheroplasts of E. coli. Heating and fast cooling of Component I for 5 min at 90° in 0.01 M phosphate (pH 7.0) changed neither its sedimentation behaviour nor its biological properties. Component II, however, was converted into biologically active single-stranded DNA by this treatment. From these results it is concluded that Component I consists of a double-stranded circular molecule in which both strands are continuous, whereas Component II consists of a double-stranded circular molecule which contains single-strand breaks." @default.
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- W1997604450 date "1966-05-01" @default.
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- W1997604450 title "Structure of the replicative form of bacteriophage ΦX174. Physico-chemical studies" @default.
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- W1997604450 doi "https://doi.org/10.1016/0005-2787(66)90187-0" @default.
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