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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997653265> ?p ?o ?g. }

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• W1997653265 abstract "Phylogenetic analysis based on 16S rDNA sequences was performed on all type strains of the 14 validly described Methylobacterium species to ascertain the genealogic relationships among these species. The results showed that type strains of Methylobacterium were divided into two monophyletic groups whose members were distinct species with sequence similarity values greater than 97.0% between any two of the members in the same group. Only M. organophilum JCM 2833T and ATCC 27886T were not divided into those two groups. In particular, strains of M. dichloromethanicum and M. chloromethanicum exhibited extremely high similarity values (99.9 and 100%, respectively) with the type strain of M. extorquens. To clarify the relationships among Methylobacterium species in more detail, phylogenetic analysis based on the 5′ end hyper-variable region of 16S rDNA (HV region), ribotyping analysis, fatty acid analysis, G+C content analysis and DNA-DNA hybridization experiments was performed on 58 strains of Methylobacterium species. Results of the ribotyping analysis and the phylogenetic analysis based on HV region sequences indicated that many Methylobacterium strains, including M. ‘organophilum’ DSM 760T, have been erroneously identified. The DNA G+C content of Methylobacterium strains were between 68.1 and 71.3%. Results of whole-cell fatty-acid profiles showed that all strains contained 18 : 1ω7c as the primary fatty acid component (82.8–90.1%), with 16 : 0 and 18 : 0 as minor components. M. dichloromethanicum DSM 6343T, M. chloromethanicum NCIMB 13688T, and M. extorquens IAM 12631T exhibited high DNA-DNA relatedness values between each other (69–80%). M. lusitanum NCIMB 13779T also showed a close relationship with M. rhodesianum DSM 5687T at DNA-DNA relatedness levels of 89–92%. According to these results, many Methylobacterium strains should be reclassified, with M. dichloromethanicum and M. chloromethanicum regarded as a synonym of M. extorquens, and M. lusitanum a synonym for M. rhodesianum." @default.
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• W1997653265 date "2005-01-01" @default.
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• W1997653265 title "Reclassification of Methylobacterium chloromethanicum and Methylobacterium dichloromethanicum as later subjective synonyms of Methylobacterium extorquens and of Methylobacterium lusitanum as a later subjective synonym of Methylobacterium rhodesianum" @default.
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• W1997653265 doi "https://doi.org/10.2323/jgam.51.287" @default.
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