FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997660008> ?p ?o ?g. }

• W1997660008 endingPage "53" @default.
• W1997660008 startingPage "43" @default.
• W1997660008 abstract "We have developed a strategy for the synthesis of novel oligodeoxynucleotide (ODN)-peptide conjugates on a scale suitable for the investigation of their potential as antisense inhibitors of gene expression. These conjugates have the 3'-terminus of the antisense oligodeoxynucleotide linked covalently to the N-terminus of a peptide. This strategy allows the preparation of conjugates containing a peptide segment designed to facilitate intracellular delivery of the antisense oligodeoxynucleotide as well as providing protection against 3'-exonuclease digestion. To illustrate the synthetic approach we describe the preparation of a series of conjugates comprising antisense oligonucleotides to human immunodeficiency virus type 1 (HIV) linked to fusion peptides derived from the HIV transmembrane glycoprotein gp41. The conjugates were prepared by the total synthesis method, in which the peptide is assembled first by the N-(fluorenylmethoxycarbonyl) (Fmoc) solid-phase methodology. This is followed by derivatization of the amino terminus by reaction with an alpha,omega-hydroxycarboxylic acid derivative which converts the terminus to a protected aliphatic hydroxy group on which standard solid phase DNA synthesis by the phosphoramidite method is performed. The purified conjugates were characterized extensively by several analytical techniques including ion spray mass spectrometry. Thermal denaturation studies showed that the interaction of the ODN-peptide conjugate with its complementary strand was similar to that of unmodified oligonucleotides. Preparation by the total synthesis method gave the purified conjugate with overall yields in the range of 6-14%." @default.
• W1997660008 created "2016-06-24" @default.
• W1997660008 creator A5028606013 @default.
• W1997660008 creator A5059755651 @default.
• W1997660008 creator A5061460270 @default.
• W1997660008 date "1995-01-01" @default.
• W1997660008 modified "2023-10-01" @default.
• W1997660008 title "Preparation and Characterization of Antisense Oligonucleotide-Peptide Hybrids Containing Viral Fusion Peptides" @default.
• W1997660008 cites W1493790412 @default.
• W1997660008 cites W1555156714 @default.
• W1997660008 cites W176140375 @default.
• W1997660008 cites W1944666585 @default.
• W1997660008 cites W1970650788 @default.
• W1997660008 cites W1971597849 @default.
• W1997660008 cites W1973588530 @default.
• W1997660008 cites W1974806453 @default.
• W1997660008 cites W1986302681 @default.
• W1997660008 cites W2002733758 @default.
• W1997660008 cites W2003326632 @default.
• W1997660008 cites W2003611716 @default.
• W1997660008 cites W2005370852 @default.
• W1997660008 cites W2006727124 @default.
• W1997660008 cites W2007332317 @default.
• W1997660008 cites W2012893162 @default.
• W1997660008 cites W2016843759 @default.
• W1997660008 cites W2018643917 @default.
• W1997660008 cites W2024809713 @default.
• W1997660008 cites W2024883068 @default.
• W1997660008 cites W2026835141 @default.
• W1997660008 cites W2026905422 @default.
• W1997660008 cites W2039450361 @default.
• W1997660008 cites W2044249986 @default.
• W1997660008 cites W2044443446 @default.
• W1997660008 cites W2045803209 @default.
• W1997660008 cites W2048235504 @default.
• W1997660008 cites W2056627100 @default.
• W1997660008 cites W2056926896 @default.
• W1997660008 cites W2064188840 @default.
• W1997660008 cites W2069464326 @default.
• W1997660008 cites W2070940062 @default.
• W1997660008 cites W2075277929 @default.
• W1997660008 cites W2078968930 @default.
• W1997660008 cites W2079242913 @default.
• W1997660008 cites W2087610022 @default.
• W1997660008 cites W2089917859 @default.
• W1997660008 cites W2092975717 @default.
• W1997660008 cites W2110917275 @default.
• W1997660008 cites W2141031088 @default.
• W1997660008 cites W2159367333 @default.
• W1997660008 cites W2170813995 @default.
• W1997660008 doi "https://doi.org/10.1021/bc00031a004" @default.
• W1997660008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7711103" @default.
• W1997660008 hasPublicationYear "1995" @default.
• W1997660008 type Work @default.
• W1997660008 sameAs 1997660008 @default.
• W1997660008 citedByCount "66" @default.
• W1997660008 countsByYear W19976600082012 @default.
• W1997660008 countsByYear W19976600082018 @default.
• W1997660008 countsByYear W19976600082021 @default.
• W1997660008 countsByYear W19976600082022 @default.
• W1997660008 countsByYear W19976600082023 @default.
• W1997660008 crossrefType "journal-article" @default.
• W1997660008 hasAuthorship W1997660008A5028606013 @default.
• W1997660008 hasAuthorship W1997660008A5059755651 @default.
• W1997660008 hasAuthorship W1997660008A5061460270 @default.
• W1997660008 hasConcept C129312508 @default.
• W1997660008 hasConcept C134306372 @default.
• W1997660008 hasConcept C185592680 @default.
• W1997660008 hasConcept C197336794 @default.
• W1997660008 hasConcept C21951064 @default.
• W1997660008 hasConcept C2776469878 @default.
• W1997660008 hasConcept C2779281246 @default.
• W1997660008 hasConcept C2779809887 @default.
• W1997660008 hasConcept C33923547 @default.
• W1997660008 hasConcept C552990157 @default.
• W1997660008 hasConcept C55493867 @default.
• W1997660008 hasConceptScore W1997660008C129312508 @default.
• W1997660008 hasConceptScore W1997660008C134306372 @default.
• W1997660008 hasConceptScore W1997660008C185592680 @default.
• W1997660008 hasConceptScore W1997660008C197336794 @default.
• W1997660008 hasConceptScore W1997660008C21951064 @default.
• W1997660008 hasConceptScore W1997660008C2776469878 @default.
• W1997660008 hasConceptScore W1997660008C2779281246 @default.
• W1997660008 hasConceptScore W1997660008C2779809887 @default.
• W1997660008 hasConceptScore W1997660008C33923547 @default.
• W1997660008 hasConceptScore W1997660008C552990157 @default.
• W1997660008 hasConceptScore W1997660008C55493867 @default.
• W1997660008 hasIssue "1" @default.
• W1997660008 hasLocation W19976600081 @default.
• W1997660008 hasLocation W19976600082 @default.
• W1997660008 hasOpenAccess W1997660008 @default.
• W1997660008 hasPrimaryLocation W19976600081 @default.
• W1997660008 hasRelatedWork W2013663885 @default.
• W1997660008 hasRelatedWork W2022383636 @default.
• W1997660008 hasRelatedWork W2063425994 @default.
• W1997660008 hasRelatedWork W2068016883 @default.
• W1997660008 hasRelatedWork W2070014241 @default.
• W1997660008 hasRelatedWork W2119446783 @default.

• next