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• W1997670864 abstract "1. To determine whether fatty acid esterification occurs at the apical surface of intestinal absorptive cells during fat absorption, we isolated and fractionated intestinal brush borders from hamsters fed pure oleic acid and from everted intestinal sacs briefly incubated with 14C-labeled oleic acid. 2. The amounts of fatty acid and triglyceride in isolated brush borders increased 3 to 5-fold after oleic acid was fed. Similarly, increased amounts of fatty acid and triglyceride were observed in the C-band fraction of disrupted brush borders. Although the C-band fraction from fasting hamsters constitutes a virtually pure preparation of microvillous membranes, this fraction was found to be contaminated with other subcellular elements when isolated from fat-fed animals. 3. When everted intestinal sacs were exposed for 30 sec to trace quantities of 14C-labeled oleic acid, almost half of the radioactivity in subsequently isolated brush borders was present as triglyceride. When these brush borders were further fractionated, glyceride specific activity was found to be low in morphologically pure C-band preparations of microvillous membranes. In contrast the AB fraction, which consists of various subcellular elements including abundant smooth endoplasmic reticulum, contained glycerides with higher specific activities than those of whole brush borders or microsomes simultaneously isolated from the remainder of the cell. 4. These findings suggest that isolated whole brush borders owe their activity for glyceride synthesis, not to any component of the microvillus itself, but to the smooth endoplasmic reticulum within the rim of attached apical cytoplasm ordinarily adherent to isolated brush borders." @default.
• W1997670864 created "2016-06-24" @default.
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• W1997670864 date "1971-06-01" @default.
• W1997670864 modified "2023-09-25" @default.
• W1997670864 title "Localization of fatty acid reesterification in the brush border region of intestinal absorptive cells" @default.
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• W1997670864 doi "https://doi.org/10.1016/0005-2736(71)90154-4" @default.
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