FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997682893> ?p ?o ?g. }

• W1997682893 endingPage "1942" @default.
• W1997682893 startingPage "1938" @default.
• W1997682893 abstract "Yeast mutants in which genes encoding subunits of the vacuolar H(+)-ATPase were interrupted were assayed for their vacuolar ATPase and proton-uptake activities. The vacuoles from the mutants lacking subunits A (72 kDa), B (57 kDa), or c (proteolipid, 16 kDa) were completely inactive in these reactions. Immunological studies revealed that in the absence of each one of those subunits the catalytic sector was not assembled. Labeling with N,N'-[14C]dicyclohexylcarbodiimide showed the presence of the proteolipid in vacuoles of mutants in which genes encoding subunits of the catalytic sectors were interrupted. No labeling was detected in the mutant in which the gene encoding the proteolipid was interrupted. We conclude that of all the ATPase subunits only the proteolipid is assembled independently and it serves as a template for the assembly of the other subunits. Site-specific mutations were generated in the gene encoding the proteolipid. All of the drastic changes and replacements gave inactive proteins. About half of the single amino acid replacements gave active proteins. Replacing glutamic acid-137 by any of several amino acids, except for aspartic acid, abolished the activity of the enzyme. Other amino acids that may function in proton conductance were changed. It was found that glycine residues may replace amino acids with exchangeable protons." @default.
• W1997682893 created "2016-06-24" @default.
• W1997682893 creator A5016351564 @default.
• W1997682893 creator A5023301230 @default.
• W1997682893 creator A5039078579 @default.
• W1997682893 creator A5085637404 @default.
• W1997682893 date "1991-03-01" @default.
• W1997682893 modified "2023-10-09" @default.
• W1997682893 title "Mutational analysis of yeast vacuolar H(+)-ATPase." @default.
• W1997682893 cites W1481801182 @default.
• W1997682893 cites W1497289365 @default.
• W1997682893 cites W1520576380 @default.
• W1997682893 cites W1534168317 @default.
• W1997682893 cites W1534442482 @default.
• W1997682893 cites W1560936920 @default.
• W1997682893 cites W1565514826 @default.
• W1997682893 cites W1566117661 @default.
• W1997682893 cites W1567029125 @default.
• W1997682893 cites W1567706221 @default.
• W1997682893 cites W1574501720 @default.
• W1997682893 cites W1576469371 @default.
• W1997682893 cites W1580841831 @default.
• W1997682893 cites W1584402017 @default.
• W1997682893 cites W1589379222 @default.
• W1997682893 cites W1592537807 @default.
• W1997682893 cites W1651085429 @default.
• W1997682893 cites W1873059855 @default.
• W1997682893 cites W1973782344 @default.
• W1997682893 cites W1989324307 @default.
• W1997682893 cites W1989883631 @default.
• W1997682893 cites W2020689496 @default.
• W1997682893 cites W2043706804 @default.
• W1997682893 cites W2044231447 @default.
• W1997682893 cites W2045890776 @default.
• W1997682893 cites W2046818599 @default.
• W1997682893 cites W2060913542 @default.
• W1997682893 cites W2091241727 @default.
• W1997682893 cites W2125452667 @default.
• W1997682893 cites W8512482 @default.
• W1997682893 doi "https://doi.org/10.1073/pnas.88.5.1938" @default.
• W1997682893 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/51141" @default.
• W1997682893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1825730" @default.
• W1997682893 hasPublicationYear "1991" @default.
• W1997682893 type Work @default.
• W1997682893 sameAs 1997682893 @default.
• W1997682893 citedByCount "143" @default.
• W1997682893 countsByYear W19976828932012 @default.
• W1997682893 countsByYear W19976828932014 @default.
• W1997682893 countsByYear W19976828932015 @default.
• W1997682893 countsByYear W19976828932018 @default.
• W1997682893 countsByYear W19976828932019 @default.
• W1997682893 crossrefType "journal-article" @default.
• W1997682893 hasAuthorship W1997682893A5016351564 @default.
• W1997682893 hasAuthorship W1997682893A5023301230 @default.
• W1997682893 hasAuthorship W1997682893A5039078579 @default.
• W1997682893 hasAuthorship W1997682893A5085637404 @default.
• W1997682893 hasBestOaLocation W19976828931 @default.
• W1997682893 hasConcept C102568950 @default.
• W1997682893 hasConcept C104292427 @default.
• W1997682893 hasConcept C104317684 @default.
• W1997682893 hasConcept C143065580 @default.
• W1997682893 hasConcept C181199279 @default.
• W1997682893 hasConcept C190062978 @default.
• W1997682893 hasConcept C23265538 @default.
• W1997682893 hasConcept C2777756961 @default.
• W1997682893 hasConcept C2779222958 @default.
• W1997682893 hasConcept C515207424 @default.
• W1997682893 hasConcept C55493867 @default.
• W1997682893 hasConcept C86803240 @default.
• W1997682893 hasConceptScore W1997682893C102568950 @default.
• W1997682893 hasConceptScore W1997682893C104292427 @default.
• W1997682893 hasConceptScore W1997682893C104317684 @default.
• W1997682893 hasConceptScore W1997682893C143065580 @default.
• W1997682893 hasConceptScore W1997682893C181199279 @default.
• W1997682893 hasConceptScore W1997682893C190062978 @default.
• W1997682893 hasConceptScore W1997682893C23265538 @default.
• W1997682893 hasConceptScore W1997682893C2777756961 @default.
• W1997682893 hasConceptScore W1997682893C2779222958 @default.
• W1997682893 hasConceptScore W1997682893C515207424 @default.
• W1997682893 hasConceptScore W1997682893C55493867 @default.
• W1997682893 hasConceptScore W1997682893C86803240 @default.
• W1997682893 hasIssue "5" @default.
• W1997682893 hasLocation W19976828931 @default.
• W1997682893 hasLocation W19976828932 @default.
• W1997682893 hasLocation W19976828933 @default.
• W1997682893 hasLocation W19976828934 @default.
• W1997682893 hasOpenAccess W1997682893 @default.
• W1997682893 hasPrimaryLocation W19976828931 @default.
• W1997682893 hasRelatedWork W1529118355 @default.
• W1997682893 hasRelatedWork W2009059248 @default.
• W1997682893 hasRelatedWork W2092603638 @default.
• W1997682893 hasRelatedWork W2105884262 @default.
• W1997682893 hasRelatedWork W2216511138 @default.
• W1997682893 hasRelatedWork W2296605487 @default.
• W1997682893 hasRelatedWork W2364427777 @default.
• W1997682893 hasRelatedWork W3141301766 @default.
• W1997682893 hasRelatedWork W37433126 @default.
• W1997682893 hasRelatedWork W4290098610 @default.

• next