FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997714902> ?p ?o ?g. }

• W1997714902 endingPage "8811" @default.
• W1997714902 startingPage "8803" @default.
• W1997714902 abstract "Brome mosaic virus (BMV) encodes two RNA replication proteins: 1a, which contains RNA capping and helicase-like domains, and 2a, which is related to polymerases. BMV 1a and 2a can direct virus-specific RNA replication in the yeast Saccharomyces cerevisiae, which reproduces the known features of BMV replication in plant cells. We constructed single amino acid point mutations at the predicted capping and helicase active sites of 1a and analyzed their effects on BMV RNA3 replication in yeast. The helicase mutants showed no function in any assays used: they were strongly defective in template recruitment for RNA replication, as measured by 1a-induced stabilization of RNA3, and they synthesized no detectable negative-strand or subgenomic RNA. Capping domain mutants divided into two groups. The first exhibited increased template recruitment but nevertheless allowed only low levels of negative-strand and subgenomic mRNA synthesis. The second was strongly defective in template recruitment, made very low levels of negative strands, and made no detectable subgenomes. To distinguish between RNA synthesis and capping defects, we deleted chromosomal gene XRN1, encoding the major exonuclease that degrades uncapped mRNAs. XRN1 deletion suppressed the second but not the first group of capping mutants, allowing synthesis and accumulation of large amounts of uncapped subgenomic mRNAs, thus providing direct evidence for the importance of the viral RNA capping function. The helicase and capping enzyme mutants showed no complementation. Instead, at high levels of expression, a helicase mutant dominantly interfered with the function of the wild-type protein. These results are discussed in relation to the interconnected functions required for different steps of positive-strand RNA virus replication." @default.
• W1997714902 created "2016-06-24" @default.
• W1997714902 creator A5012128546 @default.
• W1997714902 creator A5026899432 @default.
• W1997714902 creator A5079531852 @default.
• W1997714902 date "2000-10-01" @default.
• W1997714902 modified "2023-09-27" @default.
• W1997714902 title "Helicase and Capping Enzyme Active Site Mutations in Brome Mosaic Virus Protein 1a Cause Defects in Template Recruitment, Negative-Strand RNA Synthesis, and Viral RNA Capping" @default.
• W1997714902 cites W1568604491 @default.
• W1997714902 cites W1670572958 @default.
• W1997714902 cites W1732648959 @default.
• W1997714902 cites W1767303762 @default.
• W1997714902 cites W1833364882 @default.
• W1997714902 cites W1858596353 @default.
• W1997714902 cites W1954022543 @default.
• W1997714902 cites W1966323687 @default.
• W1997714902 cites W1972610030 @default.
• W1997714902 cites W1973782344 @default.
• W1997714902 cites W1974036147 @default.
• W1997714902 cites W1976754433 @default.
• W1997714902 cites W1981996254 @default.
• W1997714902 cites W1996320201 @default.
• W1997714902 cites W1998723532 @default.
• W1997714902 cites W2012288774 @default.
• W1997714902 cites W2012703658 @default.
• W1997714902 cites W2023413072 @default.
• W1997714902 cites W2024161219 @default.
• W1997714902 cites W2029816142 @default.
• W1997714902 cites W2037148519 @default.
• W1997714902 cites W2040091616 @default.
• W1997714902 cites W2044490480 @default.
• W1997714902 cites W2050524167 @default.
• W1997714902 cites W2050723214 @default.
• W1997714902 cites W2060483181 @default.
• W1997714902 cites W2065717236 @default.
• W1997714902 cites W2075069125 @default.
• W1997714902 cites W2081423576 @default.
• W1997714902 cites W2094533277 @default.
• W1997714902 cites W2099028920 @default.
• W1997714902 cites W2101152547 @default.
• W1997714902 cites W2105529329 @default.
• W1997714902 cites W2105973874 @default.
• W1997714902 cites W2111632147 @default.
• W1997714902 cites W2115073295 @default.
• W1997714902 cites W2125980166 @default.
• W1997714902 cites W2128298547 @default.
• W1997714902 cites W2129468535 @default.
• W1997714902 cites W2132398035 @default.
• W1997714902 cites W2135659255 @default.
• W1997714902 cites W2137294277 @default.
• W1997714902 cites W2142182685 @default.
• W1997714902 cites W2151543932 @default.
• W1997714902 cites W2153846394 @default.
• W1997714902 cites W2159791657 @default.
• W1997714902 cites W2159999829 @default.
• W1997714902 cites W2161714250 @default.
• W1997714902 cites W2164492221 @default.
• W1997714902 cites W2169690672 @default.
• W1997714902 cites W4244992019 @default.
• W1997714902 doi "https://doi.org/10.1128/jvi.74.19.8803-8811.2000" @default.
• W1997714902 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/102074" @default.
• W1997714902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10982322" @default.
• W1997714902 hasPublicationYear "2000" @default.
• W1997714902 type Work @default.
• W1997714902 sameAs 1997714902 @default.
• W1997714902 citedByCount "95" @default.
• W1997714902 countsByYear W19977149022012 @default.
• W1997714902 countsByYear W19977149022013 @default.
• W1997714902 countsByYear W19977149022014 @default.
• W1997714902 countsByYear W19977149022015 @default.
• W1997714902 countsByYear W19977149022016 @default.
• W1997714902 countsByYear W19977149022017 @default.
• W1997714902 countsByYear W19977149022018 @default.
• W1997714902 countsByYear W19977149022019 @default.
• W1997714902 countsByYear W19977149022020 @default.
• W1997714902 countsByYear W19977149022021 @default.
• W1997714902 countsByYear W19977149022022 @default.
• W1997714902 countsByYear W19977149022023 @default.
• W1997714902 crossrefType "journal-article" @default.
• W1997714902 hasAuthorship W1997714902A5012128546 @default.
• W1997714902 hasAuthorship W1997714902A5026899432 @default.
• W1997714902 hasAuthorship W1997714902A5079531852 @default.
• W1997714902 hasBestOaLocation W19977149021 @default.
• W1997714902 hasConcept C104317684 @default.
• W1997714902 hasConcept C140704245 @default.
• W1997714902 hasConcept C143065580 @default.
• W1997714902 hasConcept C153911025 @default.
• W1997714902 hasConcept C161223559 @default.
• W1997714902 hasConcept C189819185 @default.
• W1997714902 hasConcept C2522874641 @default.
• W1997714902 hasConcept C2779535198 @default.
• W1997714902 hasConcept C41258723 @default.
• W1997714902 hasConcept C54355233 @default.
• W1997714902 hasConcept C67705224 @default.
• W1997714902 hasConcept C68592252 @default.
• W1997714902 hasConcept C86803240 @default.
• W1997714902 hasConcept C95444343 @default.
• W1997714902 hasConceptScore W1997714902C104317684 @default.

• next