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• W1997759747 abstract "Significant improvement in cell growth and protein production has been achieved in Sf-9 insect cell cultures using pulse additions of multicomponent nutrient feed concentrates (Bédard et al., 1994; Chan et al., 1998). The present work focuses on investigating an alternative feeding strategy wherein the nutrients are fed in a semi continuous manner. Fed batch culture experiments were carried out to compare the two different feeding strategies, pulse and semi continuous and a process developed to achieve a cell density of 5.2 × 107 cells/mL of Sf-9 cells in a 3.5 L bioreactor. Production of recombinant protein β-galactosidase was carried out by infecting the cells with baculovirus at a MOI of 10 at cell densities of 17 × 106cells/mL. Specific productivity could be maintained at cell densities as high as 14 × 106 cells/mL. The results presented indicate that the feeding method can provide significant improvements in the performance with a reduction in the amount of total nutrients added. On-line monitoring of the culture using the capacitance probe showed that the capacitance probe can be used successfully to monitor the biomass and infection process even at higher cell densities. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 381–388, 2000." @default.
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• W1997759747 date "2000-05-20" @default.
• W1997759747 modified "2023-10-07" @default.
• W1997759747 title "Enhanced growth of sf-9 cells to a maximum density of 5.2 × 107 cells per mL and production of β-galactosidase at high cell density by fed batch culture" @default.
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• W1997759747 doi "https://doi.org/10.1002/(sici)1097-0290(20000520)68:4<381::aid-bit3>3.0.co;2-d" @default.
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