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- W1997762997 abstract "The gene encoding the bifunctional catalase-peroxidase HPI from Escherichia coli was located on a 3.8-kb Hind III fragment of the Clarke and Carbon plasmid pLC36-19 using transposon Tn 5 insertions. This fragment was subcloned into the Hind III site of pAT153 to create pBT22. The size of the insert was reduced by BAL 31 digestion of one end to an apparent minimum size for catalase expression of approx. 2.5 kb as determined by complementation and expression in maxicell strains. Further reduction in size or digestion from the opposite end inactivated the gene. The location and orientation of the promoter at the 0 kb end of the insert in pBT22 was confirmed by cloning a 320-bp Bgl II fragment into the promoter-cloning vector pKK232-8. Differences in the Southern blots of genomic DNA from a wild-type strain and a katG 17::Tn 10 mutant digested with Hinc II and probed with pBT22 confirmed that the transposon previously mapped in katG was located in the 2.5-kb coding region for HPI." @default.
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- W1997762997 date "1987-01-01" @default.
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- W1997762997 title "Physical characterization of katG, encoding catalase HPI of Escherichia coli" @default.
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- W1997762997 doi "https://doi.org/10.1016/0378-1119(87)90038-2" @default.
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