FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997770496> ?p ?o ?g. }

• W1997770496 endingPage "198" @default.
• W1997770496 startingPage "188" @default.
• W1997770496 abstract "The RNA-dependent RNA polymerase L protein of vesicular stomatitis virus (VSV), a prototypic nonsegmented negative strand (NNS) RNA virus classified into the Rhabdoviridae family, has been used to investigate the fundamental molecular mechanisms of NNS RNA viral mRNA synthesis and processing. In vitro studies on mRNA cap formation with the VSV L protein eventually led to the discovery of the unconventional mRNA capping pathway catalyzed by the guanosine 5′-triphosphatase and RNA:GDP polyribonucleotidyltransferase (PRNTase) activities. The PRNTase activity is a novel enzymatic activity, which transfers 5′-monophosphorylated (p-) RNA from 5′-triphosphorylated (ppp-) RNA to GDP to form 5′-capped RNA (GpppRNA) in a viral mRNA-start sequence-dependent manner. This unconventional capping (pRNA transfer) reaction with PRNTase can be experimentally distinguished from the conventional capping (GMP transfer) reaction with eukaryotic GTP:RNA guanylyltransferase (GTase) on the basis of the following differences in their substrate specificity for the cap formation: PRNTase uses GDP and pppRNA, but not ppRNA, whereas GTase employs GTP, but not GDP, and ppRNA. The pRNA transfer reaction with PRNTase proceeds through a covalent enzyme-pRNA intermediate with a phosphoamide bond. Hence, to prove the PRNTase activity, it is necessary to demonstrate the following consecutive steps separately: (1) the enzyme forms a covalent enzyme-pRNA intermediate, and (2) the intermediate transfers pRNA to GDP. This article describes the methods for in vitro transcription and capping with the recombinant VSV L protein, which permit detailed characterization of its enzymatic reactions and mapping of active sites of its enzymatic domains. It is expected that these systems are adaptable to rhabdoviruses and, by extension, other NNS RNA viruses belonging to different families." @default.
• W1997770496 created "2016-06-24" @default.
• W1997770496 creator A5075779892 @default.
• W1997770496 date "2013-02-01" @default.
• W1997770496 modified "2023-10-16" @default.
• W1997770496 title "In vitro capping and transcription of rhabdoviruses" @default.
• W1997770496 cites W1484881742 @default.
• W1997770496 cites W1498439983 @default.
• W1997770496 cites W1503468971 @default.
• W1997770496 cites W1509176726 @default.
• W1997770496 cites W1521436470 @default.
• W1997770496 cites W1532109734 @default.
• W1997770496 cites W1532353504 @default.
• W1997770496 cites W1596090014 @default.
• W1997770496 cites W1600954550 @default.
• W1997770496 cites W1639795491 @default.
• W1997770496 cites W1640479649 @default.
• W1997770496 cites W1645492839 @default.
• W1997770496 cites W1766852575 @default.
• W1997770496 cites W1832286915 @default.
• W1997770496 cites W1875558387 @default.
• W1997770496 cites W1904268651 @default.
• W1997770496 cites W1935059966 @default.
• W1997770496 cites W1945348710 @default.
• W1997770496 cites W1948150770 @default.
• W1997770496 cites W1967114156 @default.
• W1997770496 cites W1969542352 @default.
• W1997770496 cites W1972398797 @default.
• W1997770496 cites W1979567338 @default.
• W1997770496 cites W1979660154 @default.
• W1997770496 cites W1982098698 @default.
• W1997770496 cites W1985517822 @default.
• W1997770496 cites W1985728727 @default.
• W1997770496 cites W1990775747 @default.
• W1997770496 cites W1991640287 @default.
• W1997770496 cites W1995232699 @default.
• W1997770496 cites W2004014022 @default.
• W1997770496 cites W2005001360 @default.
• W1997770496 cites W2005385395 @default.
• W1997770496 cites W2010508642 @default.
• W1997770496 cites W2011643230 @default.
• W1997770496 cites W2014929832 @default.
• W1997770496 cites W2021968305 @default.
• W1997770496 cites W2025091176 @default.
• W1997770496 cites W2029829887 @default.
• W1997770496 cites W2031577964 @default.
• W1997770496 cites W2041245561 @default.
• W1997770496 cites W2041671233 @default.
• W1997770496 cites W2048763928 @default.
• W1997770496 cites W2049059145 @default.
• W1997770496 cites W2049221336 @default.
• W1997770496 cites W2049724155 @default.
• W1997770496 cites W2050843600 @default.
• W1997770496 cites W2058735050 @default.
• W1997770496 cites W2061270677 @default.
• W1997770496 cites W2061792416 @default.
• W1997770496 cites W2063924467 @default.
• W1997770496 cites W2064205474 @default.
• W1997770496 cites W2072644415 @default.
• W1997770496 cites W2079124918 @default.
• W1997770496 cites W2079936170 @default.
• W1997770496 cites W2081423576 @default.
• W1997770496 cites W2087538905 @default.
• W1997770496 cites W2088051028 @default.
• W1997770496 cites W2099866950 @default.
• W1997770496 cites W2103800225 @default.
• W1997770496 cites W2105778990 @default.
• W1997770496 cites W2107132989 @default.
• W1997770496 cites W2114936988 @default.
• W1997770496 cites W2119349571 @default.
• W1997770496 cites W2127026326 @default.
• W1997770496 cites W2135947735 @default.
• W1997770496 cites W2136285267 @default.
• W1997770496 cites W2146753890 @default.
• W1997770496 cites W2154480405 @default.
• W1997770496 cites W2163059846 @default.
• W1997770496 cites W2164209237 @default.
• W1997770496 cites W2166239756 @default.
• W1997770496 cites W2166783177 @default.
• W1997770496 cites W2167092519 @default.
• W1997770496 cites W2170803475 @default.
• W1997770496 cites W2171959150 @default.
• W1997770496 cites W4238099995 @default.
• W1997770496 cites W4247647064 @default.
• W1997770496 cites W4253635773 @default.
• W1997770496 cites W80209159 @default.
• W1997770496 cites W941757329 @default.
• W1997770496 doi "https://doi.org/10.1016/j.ymeth.2012.05.013" @default.
• W1997770496 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3449051" @default.
• W1997770496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22687619" @default.
• W1997770496 hasPublicationYear "2013" @default.
• W1997770496 type Work @default.
• W1997770496 sameAs 1997770496 @default.
• W1997770496 citedByCount "23" @default.
• W1997770496 countsByYear W19977704962013 @default.
• W1997770496 countsByYear W19977704962014 @default.
• W1997770496 countsByYear W19977704962015 @default.
• W1997770496 countsByYear W19977704962016 @default.

• next