FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997789657> ?p ?o ?g. }

• W1997789657 endingPage "193" @default.
• W1997789657 startingPage "185" @default.
• W1997789657 abstract "Redox transition metals have been implicated as crucial players in pathogenesis of neurodegenerative diseases. Intracellular signaling mechanism(s) responsible for oxidative stress and death in single-cell model exposed to metals has not yet been fully elucidated. The objective of the study was to determine the mechanism by which metals induced apoptosis in human peripheral blood lymphocytes (PBL).PBL were exposed to 50, 100, 250, 500, and 1,000 microM (Fe2+), (Mn2+), (Cu2+), and (Zn2+)-(SO4). Apoptotic/necrotic morphology was assessed with acridine orange/ethidium bromide staining. Further evaluations comprised production of H2O2, generation of hydroxyl radical (.OH), disruption of mitochondrial transmembrane potential (DeltaPsim), caspase-3 activation, and activation of NF-kappaB and p53 transcriptional factors.Morphologic analysis showed that 500 microM provoked maximal percentage of apoptosis (22-30% AO/EB) and minimal necrosis (3-7%), whereas low concentrations were innocuous but 1,000 microM induced mainly necrosis (>40% AO/EB). Metals generated both H2O2 and (.OH) by Fenton reaction. Hydroxyl scavengers protected PBL from metal-induced apoptosis. All metals induced mitochondrial depolarization (17-62% nonfluorescent cells) and activated caspase-3 concomitantly with apoptotic morphology (25-32% AO/EB) at 24 h, and neither NF-kappaB nor p53 transcription factor showed activation.This study provides evidence that redox-active (Fe2+), (Mn2+), (Cu2+), and (Zn2+) ion-induced apoptosis in PBL by (H2O2)/(.OH) generation, resulting in mitochondria depolarization, caspase-3 activation, and nuclear fragmentation independent of NF-kappaB and p53 transcription factors activation. Our data highlight the potential use of lymphocytes as a model to screen antioxidant strategies designed to remove H2O2/.OH associated with metal-catalyzed reactions in neurodegenerative disorders." @default.
• W1997789657 created "2016-06-24" @default.
• W1997789657 creator A5020144676 @default.
• W1997789657 creator A5042019799 @default.
• W1997789657 date "2004-05-01" @default.
• W1997789657 modified "2023-10-14" @default.
• W1997789657 title "Transition metal-induced apoptosis in lymphocytes via hydroxyl radical generation, mitochondria dysfunction, and caspase-3 activation: an in vitro model for neurodegeneration" @default.
• W1997789657 cites W1544998723 @default.
• W1997789657 cites W1589564633 @default.
• W1997789657 cites W1966855789 @default.
• W1997789657 cites W1967912613 @default.
• W1997789657 cites W1971045564 @default.
• W1997789657 cites W1974927871 @default.
• W1997789657 cites W1979070149 @default.
• W1997789657 cites W1979998867 @default.
• W1997789657 cites W1980462656 @default.
• W1997789657 cites W1982421412 @default.
• W1997789657 cites W1985613988 @default.
• W1997789657 cites W1998346093 @default.
• W1997789657 cites W1999754551 @default.
• W1997789657 cites W2004572853 @default.
• W1997789657 cites W2019279182 @default.
• W1997789657 cites W2026108006 @default.
• W1997789657 cites W2045563337 @default.
• W1997789657 cites W2048086807 @default.
• W1997789657 cites W2054293456 @default.
• W1997789657 cites W2057520074 @default.
• W1997789657 cites W2067568265 @default.
• W1997789657 cites W2070594787 @default.
• W1997789657 cites W2072568497 @default.
• W1997789657 cites W2074948983 @default.
• W1997789657 cites W2082469108 @default.
• W1997789657 cites W2082895331 @default.
• W1997789657 cites W2083301989 @default.
• W1997789657 cites W2083500052 @default.
• W1997789657 cites W2090303316 @default.
• W1997789657 cites W2094157009 @default.
• W1997789657 cites W2108771101 @default.
• W1997789657 cites W2112587738 @default.
• W1997789657 cites W2119683782 @default.
• W1997789657 cites W2121802881 @default.
• W1997789657 cites W2124684134 @default.
• W1997789657 cites W2128702649 @default.
• W1997789657 cites W2131633241 @default.
• W1997789657 cites W2132591384 @default.
• W1997789657 cites W2133186208 @default.
• W1997789657 cites W2159650080 @default.
• W1997789657 cites W2167247431 @default.
• W1997789657 cites W2168283268 @default.
• W1997789657 cites W2168558481 @default.
• W1997789657 cites W2988615119 @default.
• W1997789657 cites W4253941769 @default.
• W1997789657 cites W4296247855 @default.
• W1997789657 doi "https://doi.org/10.1016/j.arcmed.2004.01.001" @default.
• W1997789657 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15163458" @default.
• W1997789657 hasPublicationYear "2004" @default.
• W1997789657 type Work @default.
• W1997789657 sameAs 1997789657 @default.
• W1997789657 citedByCount "52" @default.
• W1997789657 countsByYear W19977896572012 @default.
• W1997789657 countsByYear W19977896572013 @default.
• W1997789657 countsByYear W19977896572014 @default.
• W1997789657 countsByYear W19977896572015 @default.
• W1997789657 countsByYear W19977896572016 @default.
• W1997789657 countsByYear W19977896572017 @default.
• W1997789657 countsByYear W19977896572018 @default.
• W1997789657 countsByYear W19977896572019 @default.
• W1997789657 countsByYear W19977896572020 @default.
• W1997789657 countsByYear W19977896572021 @default.
• W1997789657 countsByYear W19977896572023 @default.
• W1997789657 crossrefType "journal-article" @default.
• W1997789657 hasAuthorship W1997789657A5020144676 @default.
• W1997789657 hasAuthorship W1997789657A5042019799 @default.
• W1997789657 hasConcept C12519072 @default.
• W1997789657 hasConcept C12554922 @default.
• W1997789657 hasConcept C126322002 @default.
• W1997789657 hasConcept C13591479 @default.
• W1997789657 hasConcept C153911025 @default.
• W1997789657 hasConcept C185592680 @default.
• W1997789657 hasConcept C190283241 @default.
• W1997789657 hasConcept C2776151105 @default.
• W1997789657 hasConcept C2776778087 @default.
• W1997789657 hasConcept C2776925932 @default.
• W1997789657 hasConcept C2778004101 @default.
• W1997789657 hasConcept C2779102032 @default.
• W1997789657 hasConcept C2779134260 @default.
• W1997789657 hasConcept C28859421 @default.
• W1997789657 hasConcept C31573885 @default.
• W1997789657 hasConcept C4141045 @default.
• W1997789657 hasConcept C43759708 @default.
• W1997789657 hasConcept C48349386 @default.
• W1997789657 hasConcept C55493867 @default.
• W1997789657 hasConcept C71924100 @default.
• W1997789657 hasConcept C86803240 @default.
• W1997789657 hasConcept C95444343 @default.
• W1997789657 hasConceptScore W1997789657C12519072 @default.
• W1997789657 hasConceptScore W1997789657C12554922 @default.
• W1997789657 hasConceptScore W1997789657C126322002 @default.

• next