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- W1997806917 abstract "An efficient method of cross‐linking DNA to protein is described. The method is based on the incorporation of photoactive 8‐azidoadenine 2′‐deoxyribonucleotides into DNA. We have found that 8‐N 3 dATP is a substrate for E. coli DNA polymerase I and that 8‐N 3 dATP can be incorporated into plasmid pBR322 by nick‐translation. Subsequently we were able to cross‐link a set of different proteins to 8‐azido‐2′‐deoxyadenosine‐containing pBR322 by UV irradiation (366 nm). No DNA‐protein photocross‐linking was observed under the same conditions when the non‐photoactive plasmid pBR322 was used." @default.
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- W1997806917 date "1988-11-07" @default.
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- W1997806917 title "UV-induced cross-linking of proteins to plasmid pBR322 containing 8-azidoadenine 2′-deoxyribonucleotides" @default.
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- W1997806917 doi "https://doi.org/10.1016/0014-5793(88)80914-1" @default.
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