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- W1997866730 abstract "Supported lipid bilayers are a common biomimetic platform for biophysical studies of membrane proteins and the plasma membrane itself. They also hold potential for practical applications in biosensors. However, a limitation of the typical method of studying membrane proteins by reconstitution in pre-assembled bilayers is that the proper structure and function of proteins may rely on the presence of other membrane components. Toward the creation of a more comprehensive platform for studies of membrane proteins, we explored two methods of creating cell-derived supported bilayers (CDSBs). Bilayers were formed either via vesicle fusion or vesicle fusion to Langmuir-Blodgett lipid monolayers, and they were analyzed using fluorescence microscopy. The lateral mobility of lipids was assessed by fluorescence recovery after photobleaching (FRAP). In addition, impedance spectroscopy was used to measure the electrochemical properties of the CDSBs." @default.
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- W1997866730 date "2009-02-01" @default.
- W1997866730 modified "2023-09-25" @default.
- W1997866730 title "Integration of Plasma Membrane in Supported Lipid Bilayers" @default.
- W1997866730 doi "https://doi.org/10.1016/j.bpj.2008.12.1656" @default.
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