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- W1997885719 abstract "1.1. Slices of rat renal cortex were leached anaerobically at 0.5° and subsequently reincubated at 25° in either oxygenated ordinary medium, oxygenated medium containing 0.2 mM p-chloromercuribenzoic acid (PCMB) or oxygenated medium containing 0.2 mM PCMB and 10 mM ouabain.2.2. The presence of PCMB caused a greater swelling of leached slices than occurred in ordinary medium alone. During reincubation there were significant losses of water, Na+ and Cl− and significant uptakes of K+, though slices in medium with PCMB always contained more water, Na+ and Cl− and less K+ than did slices reincubated in ordinary medium.3.3. When ouabain was also present in the medium there were no appreciable differences in the water or Cl− contents of the slices but they contained more Na+ during reincubation and showed a net loss of K+.4.4. PCMB produced no convincing depression of O2 uptake in ordinary medium. However, the presence of ouabain depressed O2 uptake significantly.5.5. Slices leached anaerobically at 0.5° showed no increase in their permeability to inulin when PCMB was present.6.6. The results provide no convincing evidence that PCMB specifically inhibits any active process regulating cellular volume. They are more easily interpreted in terms of a change in membrane permeability. However, the results do support the hypothesis that the cellular volume is regulated by a mechanism other than a ouabain-sensitive Na+ pump." @default.
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- W1997885719 date "1968-12-01" @default.
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- W1997885719 title "Water and electrolyte contents of rat renal cortical slices incubated in medium containing p-chloromercuribenzoic acid or p-chloromercuribenzoic acid and ouabain" @default.
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- W1997885719 doi "https://doi.org/10.1016/0005-2736(68)90079-5" @default.
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