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- W1997910710 abstract "The microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells. Microcalorimetry is a quantitative, inexpensive, and versatile method for measuring acute toxicity. The biological response to toxicants is the inhibition of the heat production rate in cells and toxicity is expressed as the concentration of toxicant that is 50% effective in this inhibition (IC 50 ). In this paper, the effect of Pb 2+ on Rhizopus nigricans growth was investigated at 25°C. The relationship between growth rate constants ( k ) and concentration of Pb 2+ ( C ) is k = 0.04226 exp[−3.636 × 10 −5 ( C + 177.0) 2 ], and IC 50 is 47.5 μg ml −1 . These signals are readily obtained by an LKB 2277-204 heat conduction microcalorimeter. This system can therefore measure acute toxicity for a broad range of toxicants having target sites in various cellular metabolic pathways." @default.
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- W1997910710 date "1999-08-01" @default.
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- W1997910710 title "Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry" @default.
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- W1997910710 doi "https://doi.org/10.1016/s0040-6031(99)00099-4" @default.
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