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- W1997961305 abstract "On the basis of the kinetics of the peptic digestion of pig IgM, optimum conditions were determined for obtaining an Fc5μ fragment. The limited peptic digestion of pig IgM for 3 hr at 37°C in pH 4·3 buffer resulted in the production of good yields of Fab μ- and Fc5 μ-like fragments. The fragments formed by peptic digestion were fractionated by gel filtration on Sephadex G-200 and characterized by polyacrylamide disc electrophoresis, immunoelectrophoresis, double immunodiffusion, antibody activity, sedimentation constants and molecular weights. Sedimentation constants (S°20,w) were 10·6 and 3·7 and molecular weights were 324,000 and 51,800 for Fc5 μ and Fab μ respectively." @default.
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- W1997961305 date "1974-03-01" @default.
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- W1997961305 title "Peptic digestion of pig IgM" @default.
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- W1997961305 doi "https://doi.org/10.1016/0019-2791(74)90208-0" @default.
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