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- W1997971720 abstract "Cryopreserved semen samples from 10 Murrah buffalo bulls were used for sperm penetration bioassay using zona-free hamster oocytes. The samples were evaluated for sperm motility, viability and acrosome integrity. Actively motile spermatozoa recovered by the swim-up technique were capacitated using calcium ionophore A2 3 1 8 7. Mature female golden hamsters were superovulated with 50 IU PMSG followed 56 h later by 75 IU hCG. Cumulus mass, recovered by puncture of oviducts at the infundibulum region, was treated with 0.1% hyaluronidase and 0.1% trypsin to obtain zona-free oocytes.After coincubation of zona-free oocytes with capacitated buffalo spermatozoa, scoring was done as fertilization percentage and fertilization index. The correlation coefficients with conception rate were statistically significant with fertilization percentage (r = 0.588, P < 0.05) and fertilization index (r = 0.660, P < 0.01). However, conventional parameters like viability, motility and acrosome integrity showed poor correlation with conception rate." @default.
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- W1997971720 date "1992-01-01" @default.
- W1997971720 modified "2023-09-27" @default.
- W1997971720 title "Macaque monkey birth following transfer of in vitro fertilized, frozen-thawed embryos to a surrogate mother" @default.
- W1997971720 doi "https://doi.org/10.1016/0093-691x(92)90266-t" @default.
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