Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997988676> ?p ?o ?g. }
- W1997988676 endingPage "35039" @default.
- W1997988676 startingPage "35029" @default.
- W1997988676 abstract "15-Acetyldeoxynivalenol (15-AcDON) is a low molecular weight sesquiterpenoid trichothecene mycotoxin associated with Fusarium ear rot of maize and Fusarium head blight of small grain cereals. The accumulation of mycotoxins such as deoxynivalenol (DON) and 15-AcDON within harvested grain is subject to stringent regulation as both toxins pose dietary health risks to humans and animals. These toxins inhibit peptidyltransferase activity, which in turn limits eukaryotic protein synthesis. To assess the ability of intracellular antibodies (intrabodies) to modulate mycotoxin-specific cytotoxocity, a gene encoding a camelid single domain antibody fragment (V(H)H) with specificity and affinity for 15-AcDON was expressed in the methylotropic yeast Pichia pastoris. Cytotoxicity and V(H)H immunomodulation were assessed by continuous measurement of cellular growth. At equivalent doses, 15-AcDON was significantly more toxic to wild-type P. pastoris than was DON. In turn, DON was orders of magnitude more toxic than 3-acetyldeoxynivalenol. Intracellular expression of a mycotoxin-specific V(H)H within P. pastoris conveyed significant (p = 0.01) resistance to 15-AcDON cytotoxicity at doses ranging from 20 to 100 mug.ml(-1). We also documented a biochemical transformation of DON to 15-AcDON to account for the attenuation of DON cytotoxicity at 100 and 200 mug.ml(-1). The proof of concept established within this eukaryotic system suggests that in planta V(H)H expression may lead to enhanced tolerance to mycotoxins and thereby limit Fusarium infection of commercial agricultural crops." @default.
- W1997988676 created "2016-06-24" @default.
- W1997988676 creator A5007540036 @default.
- W1997988676 creator A5016428141 @default.
- W1997988676 creator A5028249593 @default.
- W1997988676 creator A5039083146 @default.
- W1997988676 creator A5042080051 @default.
- W1997988676 creator A5061881837 @default.
- W1997988676 creator A5077149161 @default.
- W1997988676 creator A5080385404 @default.
- W1997988676 creator A5090018661 @default.
- W1997988676 creator A5091303829 @default.
- W1997988676 date "2009-12-01" @default.
- W1997988676 modified "2023-09-30" @default.
- W1997988676 title "Intracellular Expression of a Single Domain Antibody Reduces Cytotoxicity of 15-Acetyldeoxynivalenol in Yeast" @default.
- W1997988676 cites W1497947649 @default.
- W1997988676 cites W1528222927 @default.
- W1997988676 cites W1577816155 @default.
- W1997988676 cites W1849061432 @default.
- W1997988676 cites W1875959162 @default.
- W1997988676 cites W1965372934 @default.
- W1997988676 cites W1971452518 @default.
- W1997988676 cites W1974125280 @default.
- W1997988676 cites W1986134620 @default.
- W1997988676 cites W1993575340 @default.
- W1997988676 cites W2005922255 @default.
- W1997988676 cites W2014604536 @default.
- W1997988676 cites W2021067723 @default.
- W1997988676 cites W2027613309 @default.
- W1997988676 cites W2043325002 @default.
- W1997988676 cites W2045321526 @default.
- W1997988676 cites W2050737769 @default.
- W1997988676 cites W2053942823 @default.
- W1997988676 cites W2054469848 @default.
- W1997988676 cites W2062814882 @default.
- W1997988676 cites W2064300027 @default.
- W1997988676 cites W2065309968 @default.
- W1997988676 cites W2066947345 @default.
- W1997988676 cites W2076582423 @default.
- W1997988676 cites W2079078533 @default.
- W1997988676 cites W2081101945 @default.
- W1997988676 cites W2086915839 @default.
- W1997988676 cites W2087171152 @default.
- W1997988676 cites W2087248514 @default.
- W1997988676 cites W2088127501 @default.
- W1997988676 cites W2088176487 @default.
- W1997988676 cites W2093165970 @default.
- W1997988676 cites W2109072371 @default.
- W1997988676 cites W2110426782 @default.
- W1997988676 cites W2113845317 @default.
- W1997988676 cites W2117994560 @default.
- W1997988676 cites W2118487113 @default.
- W1997988676 cites W2120357935 @default.
- W1997988676 cites W2124603595 @default.
- W1997988676 cites W2140846350 @default.
- W1997988676 cites W2167367711 @default.
- W1997988676 cites W2341678223 @default.
- W1997988676 cites W3002529219 @default.
- W1997988676 cites W344253813 @default.
- W1997988676 cites W4241947230 @default.
- W1997988676 cites W4249881206 @default.
- W1997988676 doi "https://doi.org/10.1074/jbc.m109.045047" @default.
- W1997988676 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2787364" @default.
- W1997988676 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19783651" @default.
- W1997988676 hasPublicationYear "2009" @default.
- W1997988676 type Work @default.
- W1997988676 sameAs 1997988676 @default.
- W1997988676 citedByCount "15" @default.
- W1997988676 countsByYear W19979886762012 @default.
- W1997988676 countsByYear W19979886762014 @default.
- W1997988676 countsByYear W19979886762015 @default.
- W1997988676 countsByYear W19979886762016 @default.
- W1997988676 countsByYear W19979886762017 @default.
- W1997988676 crossrefType "journal-article" @default.
- W1997988676 hasAuthorship W1997988676A5007540036 @default.
- W1997988676 hasAuthorship W1997988676A5016428141 @default.
- W1997988676 hasAuthorship W1997988676A5028249593 @default.
- W1997988676 hasAuthorship W1997988676A5039083146 @default.
- W1997988676 hasAuthorship W1997988676A5042080051 @default.
- W1997988676 hasAuthorship W1997988676A5061881837 @default.
- W1997988676 hasAuthorship W1997988676A5077149161 @default.
- W1997988676 hasAuthorship W1997988676A5080385404 @default.
- W1997988676 hasAuthorship W1997988676A5090018661 @default.
- W1997988676 hasAuthorship W1997988676A5091303829 @default.
- W1997988676 hasBestOaLocation W19979886761 @default.
- W1997988676 hasConcept C104317684 @default.
- W1997988676 hasConcept C109316439 @default.
- W1997988676 hasConcept C153911025 @default.
- W1997988676 hasConcept C202751555 @default.
- W1997988676 hasConcept C2776889654 @default.
- W1997988676 hasConcept C2777367657 @default.
- W1997988676 hasConcept C2778867309 @default.
- W1997988676 hasConcept C2779222958 @default.
- W1997988676 hasConcept C2781372317 @default.
- W1997988676 hasConcept C40767141 @default.
- W1997988676 hasConcept C55493867 @default.
- W1997988676 hasConcept C59822182 @default.
- W1997988676 hasConcept C79879829 @default.